Cell Line Development

• Corporate Author: SpringerLink (Online service)
• Other Authors: Al-Rubeai, Mohamed (Editor)
• Format: Electronic eBook
• Language: English
• Published: Dordrecht : Springer Netherlands, 2009.
• Series: Cell Engineering, 6
• Subjects: Medicine.; Genetic engineering.; Biochemistry.; Cell biology.; Biomedicine.; Biomedicine general.; Cell Biology.; Genetic Engineering.; Biochemistry, general.

Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when comp...

Table of Contents:

• Use of MAR Elements to Increase the Production of Recombinant Proteins
• Expression Engineering – The IE2 Promoter/Enhancer from Mouse CMV
• Defeating Randomness – Targeted Integration as a Boost for Biotechnology
• Importance of Genetic Environment for Recombinant Gene Expression
• Expression Vector Engineering for Recombinant Protein Production
• Cell Xpress™ Applications in Development and Characterization of Biopharmaceutical Recombinant Protein Producing Cell Lines
• Selection Methods for High-Producing Mammalian Cell Lines
• Engineering Mammalian Cells for Recombinant Monoclonal Antibody Production
• Engineering Cell Function by RNA Interference
• Apoptosis and Autophagy Cell Engineering
• Glycoengineering and Modeling of Protein N-Glycosylation
• Engineering the Secretory Pathway in Mammalian Cells.