Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications

• Main Authors: Pestana, Erika (Author), Belak, Sandor (Author), Diallo, Adama (Author), Crowther, John R. (Author), Viljoen, Gerrit J. (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Dordrecht : Springer Netherlands, 2010.
• Subjects: Life sciences.; Molecular biology.; Virology.; Infectious diseases.; Agriculture.; Biology > Technique.; Life Sciences.; Biological Techniques.; Infectious Diseases.; Molecular Medicine.; Biomedicine general.

This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laborator...

Table of Contents:

• Background
• Traditional PCR
• Real-Time PCR #x2013; The Basic Principles
• New Trends in the Diagnosis and Molecular Epidemiology of Viral Diseases
• Disease Diagnosis Using Real-Time PCR Specific Procedures for Important Veterinary Pathogens
• PCR Laboratory Set-up
• Analysis and Troubleshooting
• Specifications for PCR Machines.