Sample Preparation in Biological Mass Spectrometry

• Corporate Author: SpringerLink (Online service)
• Other Authors: Ivanov, Alexander R. (Editor), Lazarev, Alexander V. (Editor)
• Format: Electronic eBook
• Language: English
• Published: Dordrecht : Springer Netherlands, 2011.
• Subjects: Chemistry.; Analytical chemistry.; Mass spectrometry.; Biochemistry.; Proteomics.; Biophysics.; Biological physics.; Analytical Chemistry.; Mass Spectrometry.; Biochemistry, general.; Biomedicine general.; Biophysics and Biological Physics.

The goal and philosophy of sample preparation in biological mass spectrometry is to reveal the actual multicomponent molecular structure of a biological specimen that can be assessed by mass spectrometry tools. Unfortunately, sample preparation is often looked at as a necessary but “boring” step whi...

Table of Contents:

• Introduction: Sample Preparation – Boring but Important! Section I. Traditional and improved techniques in sample preparation for proteomics
• Section II. Methods for Improved Proteolytic Digestion
• Section III. Methods for Tissue, Cell, and Organelle Preparation and Analysis
• Section IV. 2D Gel-based Proteomics
• Section V. Sample Preparation and Analysis Techniques for Biological Fluids and Biomarker Discovery.- Section VI. Sample Preparation Methods in Plant Proteomics Section
• VII. Affinity Interaction and Biochemical Enrichment Techniques
• Section VIII. Methods for Quantitative proteomics
• Section IX. Sample Preparation in Functional Proteomics
• Section X. Characterization of Membrane Proteins.- Section XI. Methods for MALDI MS Enabled Biomedical Applications.- Section XII. Clinical Research and Applications.- Section XIII. Sample Preparation Techniques in Metabolomics and Drug Discovery
• Section XIV. Sample Preparation in Analysis or Exotic and Limited Availability Specimens
• Section XV. Sample Treatment in Biodefense, Forensics and Infectious Diseases
• Section XVI. Novel Approaches in Sample Preparation and LC-MS Analysis.