Site-directed insertion of transgenes

• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Άλλοι συγγραφείς: Renault, Sylvaine (Επιμελητής έκδοσης), Duchateau, Philippe (Επιμελητής έκδοσης)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Dordrecht : Springer Netherlands : Imprint: Springer, 2013.
• Σειρά: Topics in Current Genetics, 23
• Θέματα: Chemistry.; Gene expression.; Gene therapy.; Medical genetics.; Genetic engineering.; Genetic Engineering.; Gene Therapy.; Gene Function.; Gene Expression.

The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock...

Πίνακας περιεχομένων:

• Preface
• Section 1: Site–directed integration of transgenes
• 1 Transgene site-specific integration : troubles and solutions
• 2 :  Designing non-viral targetd integrating vectors for genome enginnering in vertebrates
• 3: Gene targeting and homologous recombination in Saccharomyces cerevisiae4: Eucaryotic homologous recombination in mammalian cells
• 5 Engineered Zinc Finger Nucleases for Targeted Genome Editing
• 6: Engineered meganucleases for genome engineering purposes
• Section3: Integration based on Site-specific recombination
• 7 : Cre/loxP, Flp/FRT systems and pluripotent stem cell lines
• 8 : Site-specific recombination using C31 Integrase
• 9 : Modified transposases used for site-directed insertion of transgenes
• 10 : Targeted Plasmid Integration into the Human Genome by Engineered Recombinases
• 11: Gene site-specific insertion in plants
• 12 Site-directed insertion of transgenes in Drosophila
• 13. Nuclease Mediated Targeted Genome Modification in Mammalian Cells
• 14: Bio-applications derived from site-directed genome modification technologies.