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|a 9789811065477
|9 978-981-10-6547-7
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|a 10.1007/978-981-10-6547-7
|2 doi
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|a TP248.13-248.65
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|a 660.6
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|a Chandran, Anandhakumar.
|e author.
|4 aut
|4 http://id.loc.gov/vocabulary/relators/aut
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|a Advancing Development of Synthetic Gene Regulators
|h [electronic resource] :
|b With the Power of High-Throughput Sequencing in Chemical Biology /
|c by Anandhakumar Chandran.
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|a 1st ed. 2018.
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|a Singapore :
|b Springer Singapore :
|b Imprint: Springer,
|c 2018.
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|a XV, 114 p. 49 illus., 44 illus. in color.
|b online resource.
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|a text
|b txt
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|a computer
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|a online resource
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|a text file
|b PDF
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|a Springer Theses, Recognizing Outstanding Ph.D. Research,
|x 2190-5053
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|a Overview of Next-Generation Sequencing Technologies and its application in Chemical Biology -- Next Generation Sequencing Studies Guide the Design of Pyrrole-Imidazole Polyamides with Improved Binding Specificity by the Addition of β-alanine -- Genome-Wide Assessment of the Binding Effects of Artificial Transcriptional Activators by Utilizing the Power of High-Throughput Sequencing -- Deciphering the genomic targets of alkylating polyamide conjugates using high-throughput sequencing.
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|a This book focuses on an "outside the box" notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)-N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence. .
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|a Biotechnology.
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|a Gene therapy.
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|a Bioorganic chemistry.
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|a Biotechnology.
|0 http://scigraph.springernature.com/things/product-market-codes/C12002
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|a Gene Therapy.
|0 http://scigraph.springernature.com/things/product-market-codes/B12020
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|a Bioorganic Chemistry.
|0 http://scigraph.springernature.com/things/product-market-codes/C19010
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|a SpringerLink (Online service)
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|t Springer eBooks
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|i Printed edition:
|z 9789811065460
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|i Printed edition:
|z 9789811065484
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|i Printed edition:
|z 9789811348990
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|a Springer Theses, Recognizing Outstanding Ph.D. Research,
|x 2190-5053
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|u https://doi.org/10.1007/978-981-10-6547-7
|z Full Text via HEAL-Link
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|a ZDB-2-CMS
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|a Chemistry and Materials Science (Springer-11644)
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