Enzyme-linked Immunosorbent Assay (ELISA) From A to Z /

• Main Authors: Hosseini, Samira (Author, http://id.loc.gov/vocabulary/relators/aut), Vázquez-Villegas, Patricia (http://id.loc.gov/vocabulary/relators/aut), Rito-Palomares, Marco (http://id.loc.gov/vocabulary/relators/aut), Martinez-Chapa, Sergio O. (http://id.loc.gov/vocabulary/relators/aut)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Singapore : Springer Singapore : Imprint: Springer, 2018.
• Edition: 1st ed. 2018.
• Series: SpringerBriefs in Forensic and Medical Bioinformatics,
• Subjects: Biomedical engineering.; Immunology.; Biophysics.; Biological physics.; Proteins .; Biotechnology.; Bioinformatics.; Biomedical Engineering and Bioengineering.; Biological and Medical Physics, Biophysics.; Protein-Ligand Interactions.; Computational Biology/Bioinformatics.

This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the...

Table of Contents:

• 1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA
• 2.General overview on applications of ELISA
• 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities
• 4. Evaluation of the detection results obtained from ELISA
• 5. Advantages, disadvantages and modifications of conventional ELISA
• 6.Common questions and answers about ELISA.