One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays

This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριος συγγραφέας: Song, Seo Woo (Συγγραφέας, http://id.loc.gov/vocabulary/relators/aut)
Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: Singapore : Springer Singapore : Imprint: Springer, 2019.
Έκδοση:1st ed. 2019.
Σειρά:Springer Theses, Recognizing Outstanding Ph.D. Research,
Θέματα:
Διαθέσιμο Online:Full Text via HEAL-Link
LEADER 04923nam a2200613 4500
001 978-981-13-8094-5
003 DE-He213
005 20191022182108.0
007 cr nn 008mamaa
008 190513s2019 si | s |||| 0|eng d
020 |a 9789811380945  |9 978-981-13-8094-5 
024 7 |a 10.1007/978-981-13-8094-5  |2 doi 
040 |d GrThAP 
050 4 |a R856-857 
050 4 |a HC79.E5 
050 4 |a GE220 
072 7 |a MQW  |2 bicssc 
072 7 |a TEC059000  |2 bisacsh 
072 7 |a MQW  |2 thema 
082 0 4 |a 610.28  |2 23 
100 1 |a Song, Seo Woo.  |e author.  |4 aut  |4 http://id.loc.gov/vocabulary/relators/aut 
245 1 0 |a One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays  |h [electronic resource] /  |c by Seo Woo Song. 
250 |a 1st ed. 2019. 
264 1 |a Singapore :  |b Springer Singapore :  |b Imprint: Springer,  |c 2019. 
300 |a XXI, 52 p. 46 illus., 40 illus. in color.  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
347 |a text file  |b PDF  |2 rda 
490 1 |a Springer Theses, Recognizing Outstanding Ph.D. Research,  |x 2190-5053 
505 0 |a Abstract -- Table of Contents -- List of Tables -- List of Figures -- Chapter 1. Introduction -- 1.1. High-Throughput Small-Volume Bioassays -- 1.2. Developmental Goal for the 'Pipetting-Free' HTS Platforms -- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs) -- Chapter 2. System Development -- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions -- 2.2. Chip and Jig Development -- 2.3. Preparation of DLPs Library -- 2.4. Decoding Microparticles -- 2.5. Statistical Analysis for Duplications -- Chapter 3. Application: Screening of Sequential Drug Combinations -- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways -- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform -- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip -- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC) -- Chapter 4. Conclusion and Discussion -- Bibliography. 
520 |a This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients' primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine. In this thesis, the author firstly describes the need and concept of 'partipetting' for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications. 
650 0 |a Biomedical engineering. 
650 0 |a Microarrays. 
650 0 |a Amorphous substances. 
650 0 |a Complex fluids. 
650 0 |a Nanotechnology. 
650 0 |a Microbiology. 
650 0 |a Biotechnology. 
650 1 4 |a Biomedical Engineering and Bioengineering.  |0 http://scigraph.springernature.com/things/product-market-codes/T2700X 
650 2 4 |a Microarrays.  |0 http://scigraph.springernature.com/things/product-market-codes/B12050 
650 2 4 |a Soft and Granular Matter, Complex Fluids and Microfluidics.  |0 http://scigraph.springernature.com/things/product-market-codes/P25021 
650 2 4 |a Nanotechnology and Microengineering.  |0 http://scigraph.springernature.com/things/product-market-codes/T18000 
650 2 4 |a Applied Microbiology.  |0 http://scigraph.springernature.com/things/product-market-codes/C12010 
650 2 4 |a Microengineering.  |0 http://scigraph.springernature.com/things/product-market-codes/C12040 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
776 0 8 |i Printed edition:  |z 9789811380938 
776 0 8 |i Printed edition:  |z 9789811380952 
776 0 8 |i Printed edition:  |z 9789811380969 
830 0 |a Springer Theses, Recognizing Outstanding Ph.D. Research,  |x 2190-5053 
856 4 0 |u https://doi.org/10.1007/978-981-13-8094-5  |z Full Text via HEAL-Link 
912 |a ZDB-2-ENG 
950 |a Engineering (Springer-11647)