One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays

• Κύριος συγγραφέας: Song, Seo Woo (Συγγραφέας, http://id.loc.gov/vocabulary/relators/aut)
• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Singapore : Springer Singapore : Imprint: Springer, 2019.
• Έκδοση: 1st ed. 2019.
• Σειρά: Springer Theses, Recognizing Outstanding Ph.D. Research,
• Θέματα: Biomedical engineering.; Microarrays.; Amorphous substances.; Complex fluids.; Nanotechnology.; Microbiology.; Biotechnology.; Biomedical Engineering and Bioengineering.; Soft and Granular Matter, Complex Fluids and Microfluidics.; Nanotechnology and Microengineering.; Applied Microbiology.; Microengineering.

This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in...

Πίνακας περιεχομένων:

• Abstract
• Table of Contents
• List of Tables
• List of Figures
• Chapter 1. Introduction
• 1.1. High-Throughput Small-Volume Bioassays
• 1.2. Developmental Goal for the 'Pipetting-Free' HTS Platforms
• 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs)
• Chapter 2. System Development
• 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions
• 2.2. Chip and Jig Development
• 2.3. Preparation of DLPs Library
• 2.4. Decoding Microparticles
• 2.5. Statistical Analysis for Duplications
• Chapter 3. Application: Screening of Sequential Drug Combinations
• 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways
• 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform
• 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip
• 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC)
• Chapter 4. Conclusion and Discussion
• Bibliography.