Επίδραση των μηχανικών ιδιοτήτων τού υποστρώματος στην κυτταρική συμπεριφορά

• Κύριος συγγραφέας: Κοζανίτη, Φωτεινή
• Άλλοι συγγραφείς: Δεληγιάννη, Δέσποινα
• Μορφή: Thesis
• Γλώσσα: English
• Έκδοση: 2017
• Θέματα: Cell material interaction; Elasticity; Αλληλεπίδραση κυττάρου-υλικού υποστρώματος; Ελαστικότητα; 571.538

The interaction between cell and material seems to have crucial importance in the field of biomaterials and tissue engineering. The cell response to surface topography, chemistry and mechanics of the substrate has been extensively investigated [1] [2] [3]. Adhesion, spreading, migration, proliferation and differentiation are the cellular activities that are mostly influenced by material properties. In the human body, the modulus of elasticity of the tissues varies between kPa, in adipose tissues and GPa, in the bones. Thus, the used biomaterials-substrates should have modulus of elasticity close to the tissues [4]. In this study, the materials-substrates have tunable modulus, as the matrix modulus is a key regulator in controlling the osteogenic potential of mesenchymal stromal cells (MSCs) [5]. Although the mechanics of the substrate have a significant effect, most of the studies, which deal with this issue [6] [7], focus only on low (kPa) Elasticity of the substrate. The aim of this study is to investigate the cellular response to substrates with a wide range of Modulus of Elasticity, but with the same topography and chemistry. The Modulus of Elasticity of the manufactured substrates varies from kPa to GPa, which is a wide range of Elasticity. Titanium, Dental Resin, PCL, PCL reinforced with CNTs substrates were manufactured with the scope of a tunable Modulus of Elasticity. The aforementioned substrates had the same chemical profile as they were coated with the same protein, fibronectin. ALP and Total Protein activity of MSCs was measured. Nuclei and cytoskeleton actin were stained. Thus, the proliferation, the differentiation and the spreading of MSCs on the substrates were investigated. Cells’ spreading was remarkably increased in the substrates with high modulus of elasticity. The proliferation of MSCs was not influenced by the mechanics of the substrate whereas the spreading was changed remarkably. The same behavior was noticed in the case of differentiation. More days of culture are indicated for further investigation of the cells’ differentiation.