| Summary: | Diboronates are proven to bind to 1,2-cis diol group containing molecules and
they are currently used in some commercial glucose biosensors. Herein, we report
a study of affinities of various carbohydrates and protein metabolites towards a
diboronate probe. The proposed method based on fluorescence could be used for
L-Glucose detection and/or quantification, where there is no known method yet.
We also examined interference by albumin in case that the method is used in real
blood samples, where we got some really encouraging results. It seems that our
probe could also be used in competitive assays between albumin and other
molecules to obtain the Kd value between them, something really useful in drug
discovery and early development, whereas screening of thousands of compounds
is substantial and cannot be supported by complicated experiments. The fastness,
feasibility and reliability of fluorescence spectroscopy as well as polarization is
highlighted in the present thesis.
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