Cell culture and confocal fluorescence imaging of natural killer‐target cell interactions in multi‐well microdevices

• Κύριος συγγραφέας: Χρηστάκου, Αθανασία
• Άλλοι συγγραφείς: Παλληκαράκης, Νικόλαος
• Μορφή: Thesis
• Γλώσσα: English
• Έκδοση: 2011
• Θέματα: Natural killer cells; Microdevices; Fluorescence imaging; Φυσικά κύτταρα δολοφόνοι; Μικροσυσκευές; Απεικόνιση φθορισμού; 616.079

The ability of culturing cells in vitro has given many advantages in biological research and has become a standard methodology in drug discovery and toxicology. However traditional culturing methods give limited possibilities comparing to microfluidic systems. In order to understand the cellular mechanisms of Natural killers against virus infected cells and tumors, we developed a method for observing in parallel, high numbers of individual Natural killer-target cell conjugates in confined regions. An important advantage of this method is that it gives the possibility to keep track of large numbers of specific conjugates in a time scale of several days. Thus live cell imaging of NK-Target cell interactions in multi-well microstructures, can offer valid statistical information about NK cells processes that can lead to a better understanding of the function and regulation of the immune system.