| spelling |
oapen-20.500.12657-230002024-03-22T19:23:35Z Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice Sarsu, Fatma Ghanim, Abdelbagi M.A. Das, Priyanka Bahuguna, Rajeev N. Kusolwa, Paul Mbogo Ashraf, Muhammed Singla-Pareek, Sneh L. Pareek, Ashwani Forster, Brian P. Ingelbrecht, Ivan Life sciences Plant breeding Agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVB Agricultural science This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance. 2020-03-18 13:36:15 2020-04-01T08:59:28Z 2020-04-01T08:59:28Z 2018 book 1007161 http://library.oapen.org/handle/20.500.12657/23000 eng application/pdf n/a 1007161.pdf https://www.springer.com/9783319773384 Springer Nature 10.1007/978-3-319-77338-4 10.1007/978-3-319-77338-4 6c6992af-b843-4f46-859c-f6e9998e40d5 39 Cham open access
|
| description |
This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.
|
