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oapen-20.500.12657-237582024-03-22T19:23:06Z Chapter 6 Optogenetic actuation, inhibition, modulation and readout for neuronal networks generating behavior in the nematode Caenorhabditis elegans de Bono, Mario Schafer, William R. Gottschalk, Alexander Hegemann, Peter Sigrist, Stephan Biophysics Genetic Engineering Neuroscience Optics Vision Restoration thema EDItEUR::P Mathematics and Science::PH Physics::PHV Applied physics::PHVN Biophysics thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAK Genetics (non-medical) thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSX Human biology Elucidating the mechanisms by which nervous systems process information and generate behavior is among the fundamental problems of biology. The complexity of our brain and plasticity of our behaviors make it challenging to understand even simple human actions in terms of molecular mechanisms and neural activity. However the molecular machines and operational features of our neural circuits are often found in invertebrates, so that studying flies and worms provides an effective way to gain insights into our nervous system. Caenorhabditis elegans offers special opportunities to study behavior. Each of the 302 neurons in its nervous system can be identified and imaged in live animals [1, 2], and manipulated transgenically using specific promoters or promoter combinations [3, 4, 5, 6]. The chemical synapses and gap junctions made by every neuron are known from electron micrograph reconstruction [1]. Importantly, forward genetics can be used to identify molecules that modulate C. elegans’ behavior. Forward genetic dis-section of behavior is powerful because it requires no prior knowledge. It allows molecules to be identified regardless of in vivo concentration, and focuses attention on genes that are functionally important. The identity and expression patterns of these molecules then provide entry points to study the molecular mechanisms and neural circuits controlling the behavior. Genetics does not provide the temporal resolution required to study neural circuit function directly. However, neural activity can be monitored using genetically encoded sensors for Ca2+ (e.g., GCaMP and cameleon) [7, 8, 9, 10] and voltage (e.g., mermaid, arclight or VSFP- Butterfly) [11, 12, 13]. In C. elegans, imaging studies have focused largely on single neurons in immobilized animals [14]. However, it is now becoming possible to image the activity of single neurons in freely moving animals, and of multiple neurons in three dimensions. Additionally, increasingly sophisticated hardware allows precise spatial control of neural activity in freely moving C. elegans, using light activated channels and pumps (see Section 6.2). From a reductionist perspective, the worm model is very exciting because it has the potential to reveal how neural circuits work in enormous detail. This potential has fostered collaborations between physicists, engineers, and neuroscientists. Here we try to convey some of the excitement in this fast moving field. 2019-11-18 23:55 2020-01-07 16:47:06 2020-04-01T09:28:17Z 2020-04-01T09:28:17Z 2013 chapter 1006384 OCN: 1135844905 9783110270716 http://library.oapen.org/handle/20.500.12657/23758 eng application/pdf n/a 46_[9783110270723 - Optogenetics] 6 Optogenetic.pdf De Gruyter Optogenetics 10.1515/9783110270723.61 10.1515/9783110270723.61 2b386f62-fc18-4108-bcf1-ade3ed4cf2f3 380c58bf-19b3-408c-a1b5-fb9410e1b5ed 7292b17b-f01a-4016-94d3-d7fb5ef9fb79 9783110270716 European Research Council (ERC) Berlin/Boston 269058 FP7 Ideas: European Research Council FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013) open access
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English
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Elucidating the mechanisms by which nervous systems process information and generate behavior is among the fundamental problems of biology. The complexity of our brain and plasticity of our behaviors make it challenging to understand even simple human actions in terms of molecular mechanisms and neural activity. However the molecular machines and operational features of our neural circuits are often found in invertebrates, so that studying flies and worms provides an effective way to gain insights into our nervous system. Caenorhabditis elegans offers special opportunities to study behavior. Each of the 302 neurons in its nervous system can be identified and imaged in live animals [1, 2], and manipulated transgenically using specific promoters or promoter combinations [3, 4, 5, 6]. The chemical synapses and gap junctions made by every neuron are known from electron micrograph reconstruction [1]. Importantly, forward genetics can be used to identify molecules that modulate C. elegans’ behavior. Forward genetic dis-section of behavior is powerful because it requires no prior knowledge. It allows molecules to be identified regardless of in vivo concentration, and focuses attention on genes that are functionally important. The identity and expression patterns of these molecules then provide entry points to study the molecular mechanisms and neural circuits controlling the behavior. Genetics does not provide the temporal resolution required to study neural circuit function directly. However, neural activity can be monitored using genetically encoded sensors for Ca2+ (e.g., GCaMP and cameleon) [7, 8, 9, 10] and voltage (e.g., mermaid, arclight or VSFP- Butterfly) [11, 12, 13]. In C. elegans, imaging studies have focused largely on single neurons in immobilized animals [14]. However, it is now becoming possible to image the activity of single neurons in freely moving animals, and of multiple neurons in three dimensions. Additionally, increasingly sophisticated hardware allows precise spatial control of neural activity in freely moving C. elegans, using light activated channels and pumps (see Section 6.2). From a reductionist perspective, the worm model is very exciting because it has the potential to reveal how neural circuits work in enormous detail. This potential has fostered collaborations between physicists, engineers, and neuroscientists. Here we try to convey some of the excitement in this fast moving field.
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46_[9783110270723 - Optogenetics] 6 Optogenetic.pdf
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46_[9783110270723 - Optogenetics] 6 Optogenetic.pdf
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46_[9783110270723 - Optogenetics] 6 Optogenetic.pdf
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title_full |
46_[9783110270723 - Optogenetics] 6 Optogenetic.pdf
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title_fullStr |
46_[9783110270723 - Optogenetics] 6 Optogenetic.pdf
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46_[9783110270723 - Optogenetics] 6 Optogenetic.pdf
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46_[9783110270723 - optogenetics] 6 optogenetic.pdf
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De Gruyter
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2019
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1799945231127281664
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