| spelling |
oapen-20.500.12657-321522021-11-12T16:32:13Z Chapter Perspectives in Glycomics and Lectin Engineering Tkac, Jan Nahalka, Jozef Gemeiner, Peter Bertok, Tomas lectins glycomics dna aptamers biosensors nanoparticles lectin peptide aptamers recombinant lectins lectins glycomics dna aptamers biosensors nanoparticles lectin peptide aptamers recombinant lectins Amino acid Carbon nanotube Glycan Glycoprotein Nucleotide bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PSB Biochemistry::PSBC Proteins This chapter would like to provide a short survey of the most promising concepts applied recently in analysis of glycoproteins based on lectins. The first part describes the most exciting analytical approaches used in the field of glycoprofiling based on integration of nanoparticles, nanowires, nanotubes, or nanochannels or using novel transducing platforms allowing to detect very low levels of glycoproteins in a label-free mode of operation. The second part describes application of recombinant lectins containing several tags applied for oriented and ordered immobilization of lectins. Besides already established concepts of glycoprofiling several novel aspects, which we think will be taken into account for future, more robust glycan analysis, are described including modified lectins, peptide lectin aptamers, and DNA aptamers with lectin-like specificity introduced by modified nucleotides. The last part of the chapter describes a novel concept of a glycocodon, which can lead to a better understanding of glycan–lectin interaction and for design of novel lectins with unknown specificities and/or better affinities toward glycan target or for rational design of peptide lectin aptamers or DNA aptamers. 2020-03-18 13:36:15 2020-04-01T13:59:41Z 2016-09-14 23:55 2020-03-18 13:36:15 2020-04-01T13:59:41Z 2016-12-31 23:55:55 2020-03-18 13:36:15 2020-04-01T13:59:41Z 2020-04-01T13:59:41Z 2014 chapter 615367 OCN: 1030815421 http://library.oapen.org/handle/20.500.12657/32152 eng application/pdf n/a 615367.pdf Springer Nature Lectins. Methods and protocols 10.1007/978-1-4939-1292-6_37 10.1007/978-1-4939-1292-6_37 6c6992af-b843-4f46-859c-f6e9998e40d5 47d753e5-083c-47f4-a2ea-42d2eb9df722 7292b17b-f01a-4016-94d3-d7fb5ef9fb79 European Research Council (ERC) 1 311532 FP7 SC39 Electrochemical LEctin and glycan biochips in FP7 Ideas: European Research Council FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013) open access
|
| description |
This chapter would like to provide a short survey of the most promising concepts applied recently in analysis of glycoproteins based on lectins. The first part describes the most exciting analytical approaches used in the field of glycoprofiling based on integration of nanoparticles, nanowires, nanotubes, or nanochannels or using novel transducing platforms allowing to detect very low levels of glycoproteins in a label-free mode of operation. The second part describes application of recombinant lectins containing several tags applied for oriented and ordered immobilization of lectins. Besides already established concepts of glycoprofiling several novel aspects, which we think will be taken into account for future, more robust glycan analysis, are described including modified lectins, peptide lectin aptamers, and DNA aptamers with lectin-like specificity introduced by modified nucleotides. The last part of the chapter describes a novel concept of a glycocodon, which can lead to a better understanding of glycan–lectin interaction and for design of novel lectins with unknown specificities and/or better affinities toward glycan target or for rational design of peptide lectin aptamers or DNA aptamers.
|
