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oapen-20.500.12657-321532021-11-12T16:21:32Z Lectins. Methods and protocols Hirabayashi, Jun lectins serial lectin-affinity chromatography lectin-probed western blot lectin technologies bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PSB Biochemistry::PSBC Proteins Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists. 2016-12-31 23:55:55 2020-03-18 13:36:15 2020-04-01T13:59:43Z 2020-04-01T13:59:43Z 2014 book 615366 OCN: 1030818405 9781493912919 http://library.oapen.org/handle/20.500.12657/32153 eng Springer Nature 10.1007/978-1-4939-1292-6 10.1007/978-1-4939-1292-6 6c6992af-b843-4f46-859c-f6e9998e40d5 d3db3b53-f5f6-42b2-8c6a-a9f39800d8a7 7292b17b-f01a-4016-94d3-d7fb5ef9fb79 9781493912919 European Research Council (ERC) 311532 FP7 SC39 FP7 Ideas: European Research Council FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013) open access
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Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists.
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