54991.pdf
Local delivery of neurotrophic factors is a pillar of neural repair strategies in the peripheral nervous system. The main disadvantage of the free growth factors is their short half‐life of few minutes. In previous studies, it was demonstrated that conjugation of various neurotrophic factors to iron...
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oapen-20.500.12657-491962021-11-23T13:51:00Z Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide Michael, Tolmasov Almog, Mara Ziv-Polat, Ofra Mandelbaum-Livnat, Mira M. Geuna, Stefano Bitan, Yifat Morano, Michela Biran, Tali Rochkind, Shimon Fregnan, Federica Reider, Evgeniy Shahar, Abraham Nissan, Moshe GDNF conjugated iron oxide nanoparticles, chitosan tube, peripheral nerve regeneration bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences Local delivery of neurotrophic factors is a pillar of neural repair strategies in the peripheral nervous system. The main disadvantage of the free growth factors is their short half‐life of few minutes. In previous studies, it was demonstrated that conjugation of various neurotrophic factors to iron oxide nanoparticles (IONP) led to stabilization of the growth factors and to the extension of their biological activity compared to the free factors. In vitro studies performed on organotypic dorsal root ganglion (DRG) cultures seeded in NVR gel (composed mainly of hyaluronic acid and laminin) revealed that the glial cell–derived neurotrophic factor (GDNF) conjugated to IONP‐enhanced early nerve fiber sprouting and accelerated the onset and progression of myelin significantly earlier than the free GDNF and other free and conjugated factors. The present article summarizes results of in vivo study, aimed to test the effect of free versus conjugated GDNF on regeneration of the rat sciatic nerve after a severe segment loss. We confirmed that nerve device enriched with a matrix with GDNF gives more successful results in term of regeneration and functional recovery in respect to the hollow tube; moreover, there are no detectable differences between free versus conjugated GDNF. 2021-06-02T10:09:10Z 2021-06-02T10:09:10Z 2017 chapter ONIX_20210602_10.5772/intechopen.68526_310 https://library.oapen.org/handle/20.500.12657/49196 eng application/pdf n/a 54991.pdf InTechOpen 10.5772/intechopen.68526 10.5772/intechopen.68526 09f6769d-48ed-467d-b150-4cf2680656a1 FP7-HEALTH-2011-two-stage 278612 open access |
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English |
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Local delivery of neurotrophic factors is a pillar of neural repair strategies in the peripheral nervous system. The main disadvantage of the free growth factors is their short half‐life of few minutes. In previous studies, it was demonstrated that conjugation of various neurotrophic factors to iron oxide nanoparticles (IONP) led to stabilization of the growth factors and to the extension of their biological activity compared to the free factors. In vitro studies performed on organotypic dorsal root ganglion (DRG) cultures seeded in NVR gel (composed mainly of hyaluronic acid and laminin) revealed that the glial cell–derived neurotrophic factor (GDNF) conjugated to IONP‐enhanced early nerve fiber sprouting and accelerated the onset and progression of myelin significantly earlier than the free GDNF and other free and conjugated factors. The present article summarizes results of in vivo study, aimed to test the effect of free versus conjugated GDNF on regeneration of the rat sciatic nerve after a severe segment loss. We confirmed that nerve device enriched with a matrix with GDNF gives more successful results in term of regeneration and functional recovery in respect to the hollow tube; moreover, there are no detectable differences between free versus conjugated GDNF. |
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