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oapen-20.500.12657-492092021-11-23T13:50:58Z Chapter Peripheral Nerve Reconstruction Using Enriched Chitosan Conduits Ronchi, Giulia Shahar, Abraham Rochkind, Shimon Reider, Evgeniy Bitan, Yifat Geuna, Stefano Viano, Nicoletta Koren, Akiva Mandelbaum-Livnat, Mira M. Morano, Michela Ziv-Polat, Ofra Biron, Tali peripheral nerve injury, chitosan guidance conduit, conduit acetylation, hyaluronic acid gel, magnetic fibrin hydrogel bic Book Industry Communication::M Medicine::MQ Nursing & ancillary services::MQW Biomedical engineering The repair of peripheral nerve traumatic lesions still represents a major cause of permanent motor and sensory impairment. In case of substance loss, a nerve guide should be used to bridge the proximal with the distal stump of the severed nerve. The effectiveness of hollow nerve guides is limited by the delay of axonal growth due to the absence of a regeneration substrate inside the conduit. To fasten up nerve regeneration, nerve guides should thus be enriched by a luminal filler. In this study, we investigated, in a 12-mm rat sciatic nerve defect experimental model, the effectiveness of chitosan-based conduits of different acetylation filled either with a hyaluronic acid gel (NVR gel) or with a magnetic fibrin hydrogel, in comparison with traditional autografts. Results showed that all types of artificial nerve conduits led to functional recovery not significantly different from autografts. By contrast, morphological and morphometrical analyses showed that the best results among nerve guides were found in medium degree of acetylation (DAII: ∼5%) chitosan conduits enriched with the NVR gel. 2021-06-02T10:09:29Z 2021-06-02T10:09:29Z 2017 chapter ONIX_20210602_10.5772/intechopen.69882_323 https://library.oapen.org/handle/20.500.12657/49209 eng application/pdf n/a 56172.pdf InTechOpen 10.5772/intechopen.69882 10.5772/intechopen.69882 09f6769d-48ed-467d-b150-4cf2680656a1 FP7-HEALTH-2011-two-stage 278612 open access
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The repair of peripheral nerve traumatic lesions still represents a major cause of permanent motor and sensory impairment. In case of substance loss, a nerve guide should be used to bridge the proximal with the distal stump of the severed nerve. The effectiveness of hollow nerve guides is limited by the delay of axonal growth due to the absence of a regeneration substrate inside the conduit. To fasten up nerve regeneration, nerve guides should thus be enriched by a luminal filler. In this study, we investigated, in a 12-mm rat sciatic nerve defect experimental model, the effectiveness of chitosan-based conduits of different acetylation filled either with a hyaluronic acid gel (NVR gel) or with a magnetic fibrin hydrogel, in comparison with traditional autografts. Results showed that all types of artificial nerve conduits led to functional recovery not significantly different from autografts. By contrast, morphological and morphometrical analyses showed that the best results among nerve guides were found in medium degree of acetylation (DAII: ∼5%) chitosan conduits enriched with the NVR gel.
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