| spelling |
oapen-20.500.12657-493332022-02-08T11:16:18Z Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology Gonçalves, Moreno Wysllas, de Freitas Oliveira Castelo, Branco Laura, Araújo Maria, Queiroz Tavares, Donato José, da Silva Júnior Thays, da Silva Rodrigues Sousa, Silva Trypanosoma cruzi, Chagas disease, cell culture, in vitro assay, metacyclogenesis bic Book Industry Communication::M Medicine::MM Other branches of medicine::MMF Pathology::MMFP Medical parasitology This chapter aims to present and discuss the main cell culture techniques used for the growth and maintenance of the different evolutionary forms of the protozoan Trypanosoma cruzi, the etiologic agent of the Chagas disease. Chagas disease is a neglected tropical disease endemic in several Latin American countries. Here, we intend to present the main difficulties, advantages, and disadvantages of using Trypanosoma cruzi cell culture in parasitic biology. Finally, we present the main research opportunities in the context of Trypanosoma cruzi parasitic biology using cell culture techniques, such as drug development, characterization of pharmacological targets, molecular markers for diagnosis, structural biology, and many other biomedical applications. 2021-06-02T10:12:22Z 2021-06-02T10:12:22Z 2019 chapter ONIX_20210602_10.5772/intechopen.84733_447 https://library.oapen.org/handle/20.500.12657/49333 eng application/pdf n/a 65997.pdf InTechOpen 10.5772/intechopen.84733 10.5772/intechopen.84733 09f6769d-48ed-467d-b150-4cf2680656a1 FP7 Health 305937 open access
|
| description |
This chapter aims to present and discuss the main cell culture techniques used for the growth and maintenance of the different evolutionary forms of the protozoan Trypanosoma cruzi, the etiologic agent of the Chagas disease. Chagas disease is a neglected tropical disease endemic in several Latin American countries. Here, we intend to present the main difficulties, advantages, and disadvantages of using Trypanosoma cruzi cell culture in parasitic biology. Finally, we present the main research opportunities in the context of Trypanosoma cruzi parasitic biology using cell culture techniques, such as drug development, characterization of pharmacological targets, molecular markers for diagnosis, structural biology, and many other biomedical applications.
|
