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oapen-20.500.12657-551022022-06-01T03:11:29Z Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology Gualdani, Roberta bic Book Industry Communication::M Medicine::MQ Nursing & ancillary services::MQP Pharmacy / dispensing bic Book Industry Communication::P Mathematics & science::PN Chemistry bic Book Industry Communication::P Mathematics & science::PN Chemistry::PNN Organic chemistry bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PSB Biochemistry bic Book Industry Communication::T Technology, engineering, agriculture::TD Industrial chemistry & manufacturing technologies Ion channels are membrane proteins that selectively allow ions to flow down their electrochemical gradient across the cellular membrane. They localize in both plasma and intracellular membranes and regulate a variety of functions such as neuronal excitability, heartbeat, muscle contraction and hormones release. Thus, understanding the molecular mechanism of ion channels function and regulation is one of the key goals of modern Biophysics. During my PhD thesis, by combining patch-clamp measurements with site-direct mutagenesis, fluorophore labeling experiments and pharmacological assays, I explored some functional and structural properties of different ion transporters: the Na+/Ca2+ exchanger (NCX); the large conductance Ca2+-voltage activated K+ channel (BK) channel; the human Transient receptor potential, member A1 (TRPA1) channel. 2022-05-31T10:21:12Z 2022-05-31T10:21:12Z 2013 book ONIX_20220531_9788866554530_386 2612-8020 9788866554530 9788866554523 9788892734708 https://library.oapen.org/handle/20.500.12657/55102 eng Premio Tesi di Dottorato application/pdf n/a 9788866554530.pdf https://books.fupress.com/isbn/9788866554530 Firenze University Press 10.36253/978-88-6655-453-0 10.36253/978-88-6655-453-0 bf65d21a-78e5-4ba2-983a-dbfa90962870 9788866554530 9788866554523 9788892734708 35 64 Florence open access
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Ion channels are membrane proteins that selectively allow ions to flow down their electrochemical gradient across the cellular membrane. They localize in both plasma and intracellular membranes and regulate a variety of functions such as neuronal excitability, heartbeat, muscle contraction and hormones release. Thus, understanding the molecular mechanism of ion channels function and regulation is one of the key goals of modern Biophysics. During my PhD thesis, by combining patch-clamp measurements with site-direct mutagenesis, fluorophore labeling experiments and pharmacological assays, I explored some functional and structural properties of different ion transporters: the Na+/Ca2+ exchanger (NCX); the large conductance Ca2+-voltage activated K+ channel (BK) channel; the human Transient receptor potential, member A1 (TRPA1) channel.
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