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oapen-20.500.12657-570462022-06-21T03:06:59Z Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana Jankowicz-Cieslak, Joanna Ingelbrecht, Ivan L. Musa Fusarium wilt TR4 mutation breeding phenotyping genotyping NGS bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PST Botany & plant sciences::PSTP Plant pathology & diseases bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PST Botany & plant sciences bic Book Industry Communication::T Technology, engineering, agriculture::TV Agriculture & farming::TVB Agricultural science Bananas are a staple food for over 500 million people and are also an important cash crop. Fusarium wilt, caused by the fungus Fusarium oxysporum f.sp. cubense, is one of the most destructive diseases of banana globally. Since the 1990s, an aggressive variant of this fungus, called Tropical Race 4 (TR4), severely affected banana plantations in Southeast Asia from where it spread to other continents, including Latin America, where the global banana export market is primarily centred. TR4 is a soil borne pathogen making the disease difficult to contain. The Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture implemented a Coordinated Research Project (CRP) ‘Efficient Screening Techniques to Identify Mutants with Disease Resistance for Coffee and Banana” (2015-2020). This CRP brought together experts from Asia, Europe and Africa in addition to experts of the Joint FAO/IAEA Centre to develop resistance against TR4 through mutation-assisted breeding. Induced mutagenesis is particularly attractive in case of banana since most cultivated bananas are seedless, thus hampering conventional cross breeding. This Open Access book is a compilation of the protocols developed under the CRP specifically for TR4. The first part covers methods for mutation induction, including the integrated use of innovative single-cell culture with mutagenesis techniques. The book also describes up-to-date phenotypic screening methods for TR4 resistance in banana under field-, greenhouse- and laboratory conditions. Finally, molecular and bioinformatics tools for genome-wide mutation discovery following Next Generation Sequencing are also described. Given the imminent threat of Fusarium Wilt TR4 on banana production globally, it is our hope and intention that the book will serve as a timely reference and guide for banana breeders and pathologists worldwide who are committed to the genetic improvement of banana for Fusarium wilt resistance. 2022-06-20T19:32:07Z 2022-06-20T19:32:07Z 2022 book ONIX_20220620_9783662649152_51 9783662649152 https://library.oapen.org/handle/20.500.12657/57046 eng application/pdf n/a 978-3-662-64915-2.pdf https://link.springer.com/978-3-662-64915-2 Springer Nature Springer 10.1007/978-3-662-64915-2 10.1007/978-3-662-64915-2 6c6992af-b843-4f46-859c-f6e9998e40d5 fc07f7d1-4c98-4b36-bd33-0bae45965006 9783662649152 Springer 187 Berlin, Heidelberg [...] International Atomic Energy Agency IAEA open access
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Bananas are a staple food for over 500 million people and are also an important cash crop. Fusarium wilt, caused by the fungus Fusarium oxysporum f.sp. cubense, is one of the most destructive diseases of banana globally. Since the 1990s, an aggressive variant of this fungus, called Tropical Race 4 (TR4), severely affected banana plantations in Southeast Asia from where it spread to other continents, including Latin America, where the global banana export market is primarily centred. TR4 is a soil borne pathogen making the disease difficult to contain. The Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture implemented a Coordinated Research Project (CRP) ‘Efficient Screening Techniques to Identify Mutants with Disease Resistance for Coffee and Banana” (2015-2020). This CRP brought together experts from Asia, Europe and Africa in addition to experts of the Joint FAO/IAEA Centre to develop resistance against TR4 through mutation-assisted breeding. Induced mutagenesis is particularly attractive in case of banana since most cultivated bananas are seedless, thus hampering conventional cross breeding. This Open Access book is a compilation of the protocols developed under the CRP specifically for TR4. The first part covers methods for mutation induction, including the integrated use of innovative single-cell culture with mutagenesis techniques. The book also describes up-to-date phenotypic screening methods for TR4 resistance in banana under field-, greenhouse- and laboratory conditions. Finally, molecular and bioinformatics tools for genome-wide mutation discovery following Next Generation Sequencing are also described. Given the imminent threat of Fusarium Wilt TR4 on banana production globally, it is our hope and intention that the book will serve as a timely reference and guide for banana breeders and pathologists worldwide who are committed to the genetic improvement of banana for Fusarium wilt resistance.
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