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oapen-20.500.12657-614822024-03-27T14:14:33Z Viruses as vectors for the delivery of gene-editing reagents Ellison, Evan E. Chamness, James C. Voytas, Daniel F. DNA viruses replicons homology-directed repair RNA viruses mutagenesis gene editing thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences A significant challenge for plant gene editing is the delivery of editing reagents to germline or regenerable cells to recover heritable genetic modifications. Reagent delivery using biolistics or Agrobacterium is only possible with a limited range of species and genotypes, and inefficient editing or lengthy tissue culture steps further limit throughput. Viruses are natural vectors for nucleic acids, and both DNA and RNA plant viruses have been engineered to extend or replace conventional vectors for delivery of gene editing reagents. Here, we review aspects of viral biology essential for engineering vectors, highlight landmark studies using viruses to overcome traditional limitations in gene editing, and outline important considerations for the use of viral vectors in new systems or for new targets. Motivated by fundamental differences in both their infection modes and utility as vectors, DNA and RNA viruses are treated separately. 2023-02-27T12:21:05Z 2023-02-27T12:21:05Z 2021 chapter ONIX_20230227_9781786769213_8 9781786769213 https://library.oapen.org/handle/20.500.12657/61482 eng Burleigh Dodds Series in Agricultural Science application/pdf Attribution 4.0 International 9781786769213_web.pdf Burleigh Dodds Science Publishing Burleigh Dodds Science Publishing 10.19103/AS.2020.0082.28 10.19103/AS.2020.0082.28 9f8f6c63-e2ae-40b8-8aac-316abb377d6a d8a9624d-2c03-48b6-9cea-4fe3be38f995 9781786769213 Burleigh Dodds Science Publishing 26 Cambridge [...] University of Minnesota UM open access
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A significant challenge for plant gene editing is the delivery of editing reagents to germline or regenerable cells to recover heritable genetic modifications. Reagent delivery using biolistics or Agrobacterium is only possible with a limited range of species and genotypes, and inefficient editing or lengthy tissue culture steps further limit throughput. Viruses are natural vectors for nucleic acids, and both DNA and RNA plant viruses have been engineered to extend or replace conventional vectors for delivery of gene editing reagents. Here, we review aspects of viral biology essential for engineering vectors, highlight landmark studies using viruses to overcome traditional limitations in gene editing, and outline important considerations for the use of viral vectors in new systems or for new targets. Motivated by fundamental differences in both their infection modes and utility as vectors, DNA and RNA viruses are treated separately.
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