Regulators of G-protein signaling. Part A /

• Άλλοι συγγραφείς: Siderovski, David P.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam ; Boston : Elsevier Academic Press, 2004.
• Σειρά: Methods in enzymology ; volume 389.
• Θέματα: G proteins.; Intracellular Signaling Peptides and Proteins.; GTP-Binding Protein Regulators.; RGS Proteins > analysis.; Protéines G.; G-proteïnen.; Signalen.; Electronic books.

 This volume is an in-depth treatment of G-Protein Signaling, Part A, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subunits, Mouse models of RGS protein action, Methods of RGS protein inhibition, and G-protein regulators of model organisms. Table of Contents -Expression and post-translational modification -Assays of GAP activity and allosteric control -Electrophysiological methods and RGS-insenstitive Ga subunits -Mouse Models of RGS protein action -Methods of RGS protein inhibition -G-protein regulators of model organisms. 

 Cover -- Table of Contents -- Contributors to Volume 389 -- Preface -- Volumes in Series -- Section I: General Methods of RGS Protein Analysis Subsection A. Expression and Post-translational Modification -- Chapter 1. Quantitative Real-Time Polymerase Chain Reaction Measurement of Regulators of G-Protein Signaling mRNA Levels in Mouse Tissues -- Chapter 2. Regulation of Chemokine-Induced Lymphocyte Migration by RGS Proteins -- Chapter 3. Role of Palmitoylation in RGS Protein Function -- Subsection B: Assays of RGS Box Activity and Allosteric Control -- Chapter 4. Fluorescence-Based Assays for RGS Box Function -- Chapter 5. Application of RGS Box Proteins to Evaluate G-Protein Selectivity in Receptor-Promoted Signaling -- Chapter 6. Allosteric Regulation of GAP Activity by Phospholipids in Regulators of G-Protein Signaling -- Chapter 7. Assays of RGS Protein Modulation by Phosphatidylinositides and Calmodulin -- Subsection C. Electrophysiological Methods and RGS-Insensitive Galpha Subunits -- Chapter 8. Analyses of RGS Protein Control of Agonist- Evoked Ca2+ Signaling -- Chapter 9. Measuring the Modulatory Effects of RGS Proteins on GIRK Channels -- Chapter 10. Assays for G-Protein-Coupled Receptor Signaling Using RGS-Insensitive Galpha Subunits -- Chapter 11. Use of RGS-Insensitive Galpha Subunits to Study Endogenous RGS Protein Action on G-Protein Modulation of N-Type Calcium Channels in Sympathetic Neurons -- Chapter 12. Endogenous RGS Proteins Regulate Presynaptic and Postsynaptic Function: Functional Expression of RGS-Insensitive Galpha Subunits in Central Nervous System Neurons -- Subsection D. Mouse Models of RGS Protein Action -- Chapter 13. In Situ Hybridization Analysis of RGS mRNA Regulation and Behavioral Phenotyping of RGS Mutant Mice -- Chapter 14. RGS-Insensitive G-Protein Mutations to Study the Role of Endogenous RGS Proteins -- Subsection E. Methods of RGS Protein Inhibition -- Chapter 15. Ribozyme- and siRNA-Mediated Suppression of RGS-Containing RhoGEF Proteins -- Chapter 16. Structure-Based Design, Synthesis, and Activity of Peptide Inhibitors of RGS4 GAP Activity -- Chapter 17. Yeast-Based Screening for Inhibitors of RGS Proteins -- Section II: G-Protein Regulators of Model Organisms -- Chapter 18. Genetic Analysis of RGS Protein Function in Caenorhabditis elegans -- Chapter 19. Purification and In Vitro Functional Analysis of the Arabidopsis thaliana Regulator of G-Protein Signaling-1 -- Chapter 20. AtRGS1 Function in Arabidopsis thaliana -- Chapter 21. Identification and Functional Analysis of the Drosophila Gene loco -- Chapter 22. Analysis of the Roles of Pins and Heterotrimeric G Proteins in Asymmetric Division of Drosophila Neuroblasts -- Chapter 23. Mathematical Modeling of RGS and G-Protein Regulation in Yeast -- Chapter 24. Identification of Yeast Pheromone Pathway Modulators by High-Throughput Agonist Response Profiling of a Yeast Gene Knockout Strain Collection -- Author Index -- Subject Index -- Last Page.