RNA interference /

• Άλλοι συγγραφείς: Engelke, David R. (David Ross), Rossi, John J.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego : Elsevier Academic Press, ©2005.
• Σειρά: Methods in enzymology ; v. 392.
• Θέματα: RNA > Handbooks, manuals, etc.; RNA editing > Handbooks, manuals, etc.; Gene silencing > Handbooks, manuals, etc.; Small interfering RNA > Handbooks, manuals, etc.; ARN.; ARN > Édition.; Inactivation génique.; Petit ARN interférent.; SCIENCE > Life Sciences > Molecular Biology.; Gene silencing.; RNA.; RNA editing.; Small interfering RNA.; RNA Interference [MESH]; RNA [MESH]; RNA Editing [MESH]; Gene Silencing [MESH]; RNA, Small Interfering [MESH]; Electronic books.; Handbooks and manuals.

The newest volume in the critically acclaimed laboratory standard, Methods in Enzymology.

Πίνακας περιεχομένων:

• Cover
• front cover
• copyright
• Table of Contents
• Contributors to Volume 392
• Methods in Enzymology
• [1] Transgene-Induced RNA Interference as a Tool for Plant Functional Genomics
• [2] Constructs and Methods for Hairpin RNA-Mediated Gene Silencing in Plants
• [3] RNA Interference in Caenorhabditis elegans
• [4] High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells
• [5] Mechanistic Insights Aid Computational Short Interfering RNA Design
• [6] Novel Methods for Expressing RNA Interference in Human Cells
• [7] Delivery of Small Interfering RNA to Mammalian Cells in Culture by Using Cationic Lipid/Polymer-Based Transfection Reagents
• [8] Subcellular Distribution of Small Interfering RNA: Directed Delivery Through RNA Polymerase III Expression Cassettes and Localization by In Situ Hybridization
• [9] Viral Delivery of Recombinant Short Hairpin RNAs
• [10] Targeting Cellular Genes with PCR Cassettes Expressing Short Interfering RNAs
• [11] Use of Short Hairpin RNA Expression Vectors to Study Mammalian Neural Development
• [12] Analysis of T-Cell Development by Using Short Interfering RNA to Knock Down Protein Expression
• [13] Lentiviral Vector Delivery of Recombinant Small Interfering RNA Expression Cassettes
• [14] Paradigms for Conditional Expression of RNA Interference Molecules for Use Against Viral Targets
• [15] High-Throughput RNA Interference Strategies for Target Discovery and Validation by Using Synthetic Short Interfering RNAs: Functional Genomics Investigations of Biological Pathways
• [16] RNA Interference In Vivo: Toward Synthetic Small Inhibitory RNA-Based Therapeutics
• [17] Labeling and Characterization of Small RNAs Associated with the RNA Interference Effector Complex RITS
• [18] RNA Interference Spreading in C. elegans
• [19] Human Dicer: Purification, Properties, and Interaction with PAZ PIWI Domain Proteins
• [20] Delivery of siRNA and siRNA Expression Constructs to Adult Mammals by Hydrodynamic Intravascular Injection
• [21] Analysis of Short Interfering RNA Function in RNA Interference by Using Drosophila Embryo Extracts and Schneider Cells
• [22] Use of RNA Polymerase II to Transcribe Artificial MicroRNAs
• [23] Adeno-Associated Virus Vectors for Short Hairpin RNA Expression
• [24] Simple, Robust Strategies for Generating DNA-Directed RNA Interference Constructs
• Author Index
• Subject Index
• Last Page.