DNA microarrays. Volume 410, Part A /
Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist th...
| Άλλοι συγγραφείς: | , |
|---|---|
| Μορφή: | Ηλ. βιβλίο |
| Γλώσσα: | English |
| Έκδοση: |
Amsterdam ; San Diego :
Elsevier/Academic Press,
©2006.
|
| Σειρά: | Methods in enzymology ;
v. 410. |
| Θέματα: | |
| Διαθέσιμο Online: | Full Text via HEAL-Link |
Πίνακας περιεχομένων:
- pt. A. Array platforms and wet-bench protocols
- pt. B. Databases and statistics.
- Section I Array Platforms
- Chapter 1: The Affymetrix GeneChipreg Platform: An Overview
- Introduction
- GeneChip Microarrays, a Flexible Platform
- Array Manufacturing
- Array Design
- Target Preparation
- GeneChip Instrument Components and Associated Assay Steps
- Image and Data Analysis
- Current Applications
- Advancing the Future of Genomics
- Acknowledgments
- References
- Chapter 2: The Agilent In Situ-Synthesized Microarray Platform
- Introduction
- Technology
- Applications
- Methods Descriptions
- Array Design
- Sample Isolation, Labeling, and Quality Control
- Array Hybridization and Scanning
- Data Extraction
- Array Design
- Process Description
- Sample Isolation, Labeling, and Quality Control
- Array Hybridization and Scanning
- Data Extraction
- References
- Chapter 3: Illumina Universal Bead Arrays
- Introduction
- Material and Methods
- Results and Discussion
- Conclusion
- Acknowledgments
- References
- Chapter 4: Microarray Oligonucleotide Probes
- The Case for Oligonucleotide Probes
- Considerations for Oligonucleotide Probe Design
- Microarray Production and Hybridization Protocols
- Practical Considerations in Probe Sequence Design, a Case Study
- Employment and Postprocessing
- Conclusion
- Locations of Probe Sequence Target Regions: Discrimination of Highly Similar Targets
- In Situ Synthesis vs Deposition of Presynthesized Oligonucleotides
- Thermodynamic Modeling of Microarray Probe Hybridization
- Outlook
- Supplement
- Acknowledgments
- References
- Chapter 5: Automated Liquid Handling and High-Throughput Preparation of Polymerase Chain Reaction-Amplified DNA for Microarray Fabrication
- Introduction
- Overview
- Methods
- Summary
- Acknowledgments
- References
- Chapter 6: The Printing Process: Tips on Tips
- Introduction
- The Printing Process and Equipment
- Printing Pin Technology Options
- Critical Parameters and Troubleshooting
- Quality Control Testing
- Future Developments
- Acknowledgment
- References
- Chapter 7: Making and Using Spotted DNA Microarrays in an Academic Core Laboratory
- Introduction
- Technologies and Services Provided by the Keck Microarray Resource
- Generic Glass Slide Microarray Printing
- Genomic Solutions OmniGrid 100 Microarrayer Print Settings
- Materials and General Settings for cDNA and Oligonucleotide Printing on In-House PLL-Coated Slides
- Quality Control Parameters for DNA Microarray Printing and Analysis Using Spotted Glass Slides
- Labeling and Hybridization Protocols Employed by the Keck Microarray Resource
- General Considerations Regarding RNA Samples, Labeling, and Hybridization Strategies
- Indirect Amino-Allyl dUTP Target Labeling, Monofunctional Dye Conjugation, and cDNA and Oligonucleotide Array Hybridization Protocols
- Genisphere 3DNA Dendrimer Labeling and Oligonucleotide Array Hybridization
- tidtid6.
