DNA microarrays. Volume 410, Part A /

• Άλλοι συγγραφείς: Kimmel, Alan R., Oliver, Brian, 1959-
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam ; San Diego : Elsevier/Academic Press, ©2006.
• Σειρά: Methods in enzymology ; v. 410.
• Θέματα: DNA microarrays.; DNA.; Oligonucleotide Array Sequence Analysis > methods.; Gene Expression Profiling > methods.; Electronic books.

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist th...

Πίνακας περιεχομένων:

• pt. A. Array platforms and wet-bench protocols
• pt. B. Databases and statistics.
• Section I Array Platforms
• Chapter 1: The Affymetrix GeneChipreg Platform: An Overview
• Introduction
• GeneChip Microarrays, a Flexible Platform
• Array Manufacturing
• Array Design
• Target Preparation
• GeneChip Instrument Components and Associated Assay Steps
• Image and Data Analysis
• Current Applications
• Advancing the Future of Genomics
• Acknowledgments
• References
• Chapter 2: The Agilent In Situ-Synthesized Microarray Platform
• Introduction
• Technology
• Applications
• Methods Descriptions
• Array Design
• Sample Isolation, Labeling, and Quality Control
• Array Hybridization and Scanning
• Data Extraction
• Array Design
• Process Description
• Sample Isolation, Labeling, and Quality Control
• Array Hybridization and Scanning
• Data Extraction
• References
• Chapter 3: Illumina Universal Bead Arrays
• Introduction
• Material and Methods
• Results and Discussion
• Conclusion
• Acknowledgments
• References
• Chapter 4: Microarray Oligonucleotide Probes
• The Case for Oligonucleotide Probes
• Considerations for Oligonucleotide Probe Design
• Microarray Production and Hybridization Protocols
• Practical Considerations in Probe Sequence Design, a Case Study
• Employment and Postprocessing
• Conclusion
• Locations of Probe Sequence Target Regions: Discrimination of Highly Similar Targets
• In Situ Synthesis vs Deposition of Presynthesized Oligonucleotides
• Thermodynamic Modeling of Microarray Probe Hybridization
• Outlook
• Supplement
• Acknowledgments
• References
• Chapter 5: Automated Liquid Handling and High-Throughput Preparation of Polymerase Chain Reaction-Amplified DNA for Microarray Fabrication
• Introduction
• Overview
• Methods
• Summary
• Acknowledgments
• References
• Chapter 6: The Printing Process: Tips on Tips
• Introduction
• The Printing Process and Equipment
• Printing Pin Technology Options
• Critical Parameters and Troubleshooting
• Quality Control Testing
• Future Developments
• Acknowledgment
• References
• Chapter 7: Making and Using Spotted DNA Microarrays in an Academic Core Laboratory
• Introduction
• Technologies and Services Provided by the Keck Microarray Resource
• Generic Glass Slide Microarray Printing
• Genomic Solutions OmniGrid 100 Microarrayer Print Settings
• Materials and General Settings for cDNA and Oligonucleotide Printing on In-House PLL-Coated Slides
• Quality Control Parameters for DNA Microarray Printing and Analysis Using Spotted Glass Slides
• Labeling and Hybridization Protocols Employed by the Keck Microarray Resource
• General Considerations Regarding RNA Samples, Labeling, and Hybridization Strategies
• Indirect Amino-Allyl dUTP Target Labeling, Monofunctional Dye Conjugation, and cDNA and Oligonucleotide Array Hybridization Protocols
• Genisphere 3DNA Dendrimer Labeling and Oligonucleotide Array Hybridization
• tidtid6.