Stem cell tools and other experimental protocols /

• Other Authors: Klimanskaya, Irina, Lanza, R. P. (Robert Paul), 1956-
• Format: eBook
• Language: English
• Published: Amsterdam ; Boston : Elsevier Academic Press, ©2006.
• Series: Methods in enzymology ; v. 420.
• Subjects: Stem cells.; Stem cells > Research > Handbooks, manuals, etc.; Tissue engineering.; SCIENCE > Life Sciences > Developmental Biology.; Stem cells > Research.; Stamcellen.; Onderzoek.; Stem Cells [MESH]; Embryo Research [MESH]; Tissue Engineering [MESH]; Electronic books.; Handbooks and manuals.

This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often ha...

Table of Contents:

• In vitro experimentation and research tools: Human embryo culture / A. Mercader [and others]
• Characterization and evaluation of human embryonic stem cells / C. Xu [and others]
• Feeder-free culture of human embryonic stem cells / M. Amit [and others]
• Transgene expression and RNA interference in embryonic stem cells / H. Zaehres [and others]
• Lentiviral vector-mediated gene delivery into human embryonic stem cells / M. Gropp [and others]
• The use of retroviral vectors for gene transfer into hematopoietic stem cells / J. Holst [and others]
• Engineering embryonic stem cells with recombinase systems / F. Schnutgen [and others]
• Gene trapping in embryonic stem cells / W.L. Stanford [and others]
• Genechips in stem cell research / J. Hipp [and others]
• Microarray analysis of stem cells and differentiation / H.Y. Chang [and others]
• Purification of hematopoietic stem cells using the side population / K.K. Lin [and others]
• Cellular reprogramming / S. Agarwal [and others]
• Tissue engineering and regenerative medicine: Tissue engineering using adult stem cells / D. Eberli [and others]
• Tissue engineering using human embryonic stem cells / S. Cohen [and others]
• Engineering cardiac tissue from embryonic stem cells / X. Guo [and others]
• Mesenchymal stem cells and tissue engineering / N.W. Marion [and others]
• Bone reconstruction with bone marrow stromal cells / W. Liu [and others]
• Engineering three-dimensional tissue structures using stem cells / J. Zoldan [and others]
• Immunogenicity of embryonic stem cells and their progeny / M. Drukker [and others]
• Manufacturing considerations for clinical uses of therapies derived from stem cells / D.J. Weber.