Advanced bacterial genetics : use of transposons and phage for genomic engineering /

• Other Authors: Hughes, Kelly Thomas, Maloy, Stanley R.
• Format: eBook
• Language: English
• Published: Amsterdam ; Boston : Elsevier / Academic Press, ©2007.
• Series: Methods in enzymology ; v. 421.
• Subjects: Bacterial genetics.; Transposons.; Bacteriophages.; SCIENCE > Life Sciences > Biochemistry.; Genetische manipulatie.; Bacteriën.; Transposonen.; Bacteriofagen.; Genoom.; Genomica.; Mutagenesis.; Bacteria > genetics.; DNA, Bacterial.; Electronic books.

 A volume of indispensable methods for the use of transposons and phage in genomic engineering. 

 Strain collections and genetic nomenclature / Stanley R. Maloy and Kelly T. Hughes -- Use of antibiotic-resistant transposons for mutagenesis / Stanley R. Maloy -- In vivo mutagenesis using EZ-Tn5 / John R. Kirby -- Identification of essential genes in bacteria / David R. Hillyard and Michael J. Reed -- Isolation of transposon insertions / Stanley R. Maloy -- Localized mutagenesis / Stanley R. Maloy -- Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons / Kelly T. Hughes -- Target-directed proteolysis in vivo / Markus Eser [and others] -- Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering / Beth Traxler and Eliora Gachelet -- Using genomic microarrays to study insertional/transposon mutant libraries / David N. Baldwin and Nina R. Salama -- Screening transposon mutant libraries using full-genome oligonucleotide microarrays / Kelly M. Winterberg and William S. Reznikoff -- Creating recombination-activated genes and sequence-defined mutant libraries using transposons / Larry Gallagher [and others] -- Use of operon and gene fusions to study gene regulation in Salmonella / Kelly T. Hughes and Stanley R. Maloy -- Genomic screening for regulatory genes using the T-POP transposon / Changhan Lee [and others] -- Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond / James A. Sawitzke [and others] -- [lambda]-red genetic engineering in Salmonella enterica serovar Typhimurium / Joyce E. Karlinsey -- Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements / Nathalie Garcia-Russell, Samantha S. Orchard, and Anca M. Segall -- Dissecting nucleic acid-protein interactions using challenge phage / Stanley R. Maloy and Jeffrey Gardner -- Mud-P22 / Jenny A. Crain and Stanley R. Maloy -- Phage metagenomics / Veronica Casas and Forest Rowher.