Ribonucleases. Volume 341, Part A /
This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and...
| Άλλοι συγγραφείς: | |
|---|---|
| Μορφή: | Ηλ. βιβλίο |
| Γλώσσα: | English |
| Έκδοση: |
San Diego :
Academic Press,
©2001.
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| Σειρά: | Methods in enzymology ;
volume 341, pt. A. |
| Θέματα: | |
| Διαθέσιμο Online: | Full Text via HEAL-Link |
Πίνακας περιεχομένων:
- Front Cover; Ribonucleases; Copyright Page; Table of Contents; Contributors to Volume 341; Preface; Volume in Series; Section I: Ribonuclease Classification and Review; Chapter 1. A Natural Classification of Ribonucleases; Chapter 2. The Ribonuclease T1 Family; Chapter 3. Ribonuclease T2; Chapter 4. The Ribonuclease P Family; Section II: Ribonuclease Assays; Chapter 5. Fast, Facile, Hypersensitive Assays for Ribonucleolytic Activity; Chapter 6. Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method after Isoelectric Focusing.
- Chapter 7. Gel Renaturation Assay for RibonucleasesChapter 8. Analysis of Ribonucleases Following Gel Electrophoresis; Chapter 9. Ribonuclease Assays Utilizing Toluidine Blue Indicator Plates, Methylene Blue, or Fluorescence Correlation Spectroscopy; Chapter 10. Ribonuclease Activities of Trypanosome RNA Editing Complex Directed to Cleave Specifically at a Chosen Site; Chapter 11. Ribonuclease YI*, RNA Structure Studies, and Variable Single-Strand Specificities of RNases; Section III: Secreted Ribonucleases.
- Chapter 12. Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding StudiesChapter 13. Purification of Engineered Human Pancreatic Ribonuclease; Chapter 14. Degradation of Double-Stranded RNA by Mammalian Pancreatic-Type Ribonucleases; Chapter 15. Seminal Ribonuclease: Preparation of Natural and Recombinant Enzyme, Quaternary Isoforms, Isoenzymes, Monomeric Forms; Assay for Selective Cytotoxicity of the Enzyme; Chapter 16. Angiogenin; Chapter 17. Eosinophil-Derived Neurotoxin; Chapter 18. Eosinophil Cationic Protein; Chapter 19. Deciphering the Mechanism of RNase T1.
- Chapter 20. Mitogillin and Related Fungal RibotoxinsChapter 21. RNase U2 and a-Sarcin: A Study of Relationships; Chapter 22. Secretory Acid Ribonucleases from Tomato, Lycopersicon esculentum Mill.; Chapter 23. Leczyme; Section IV: Ribonucleases H; Chapter 24. Prokaryotic Type 2 RNases H; Chapter 25. RNase H1 of Saccharomyces cerevisiae: Methods and Nomenclature; Chapter 26. Ribonucleases H of the Budding Yeast, Saccharomyces cerevisiae; Chapter 27. Human RNases H; Chapter 28. Assays for Retroviral RNase H; Section V: Synthetic Ribonucleases.
- Chapter 29. Sequence-Selective Artificial RibonucleasesChapter 30. RNA Cleavage by 1,4-Diazabicyclo[2.2.2]- octane-Imidazole Conjugates; Chapter 31. Preparation and Use of ZFY-6 Zinc Finger Ribonuclease; Section VI: Ribonucleolytic Nucleic Acids; Chapter 32. RNA Cleavage by the 10-23 DNA Enzyme; Chapter 33. Leadzyme; Chapter 34. Hammerhead Ribozyme Structure and Function in Plant RNA Replication; Chapter 35. Kinetic Analysis of Bimolecular Hepatitis delta Ribozyme; Chapter 36. Catalytic and Structural Assays for the Hairpin Ribozyme; Chapter 37. Intracellular Applications of Ribozymes.
