Chromatin and chromatin remodeling enzymes.

Chromatin and chromatin remodeling enzymes. Part A /

DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodellng enzymes play an important role in gene transcription. *Histone Bioinformatics *Biochemistry of histones, nucleosomes and chromatin *Molecular cytology of chromatin functions.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Άλλοι συγγραφείς: Allis, C. David, Wu, Carl
Μορφή: Ηλ. βιβλίο
Γλώσσα:English
Έκδοση: Amsterdam ; Boston : Elsevier Academic Press, ©2004.
Σειρά:Methods in enzymology ; v. 375.
Θέματα:
Chromatin.
SCIENCE > Life Sciences > Biochemistry.
Biofísica.
Chromatin > physiology [MESH]
Chromatin > enzymology [MESH]
Cytological Techniques [MESH]
Electronic books.
Διαθέσιμο Online:Full Text via HEAL-Link
Πίνακας περιεχομένων:
  • 1. Mining core histone sequences from public protein databases
  • 2. Reconstitution of nucleosome core particles from recombinant histones and DNA
  • 3. Preparation and crystallization of nucleosome core particle
  • 4. Creating designer histones by native chemical ligation
  • 5. Two-dimensional gel analysis of histones and other H2AX-related methods
  • 6. Assembly of yeast chromatin using ISWI complexes
  • 7. Reconstitution of yeast chromatin using yNap1p
  • 8. DNA synthesis-dependent and -independent chromatin assembly pathways in Xenopus egg extracts
  • 9. Preparation of defined mononucleosomes, dinucleosomes, and nucleosome arrays in vitro and analysis of transcription factor binding
  • 10. Purification of native, defined chromatin segments
  • 11. Purification of defined chromosomal domains
  • 12. Probing core histone tail-DNA interactions in a model dinucleosome system
  • 13. Site-directed histone-DNA contact mapping for analysis of nucleosome dynamics
  • 14. Site-specific attachment of reporter compounds to recombinant histones
  • 15. Histone MacroH2A purification and nucleosome reconstitution
  • 16. Analysis of histone variant H2A.Z localization and expression during early development
  • 17. Histone variant CENP-A purification, nucleosome reconstitution
  • 18. Immunological analysis and purification of centromere complex
  • 19. Purification and analyses of histone H1 variants and H1 posttranslational modifications
  • 20. HMGA proteins: isolation, biochemical modifications, and nucleosome interactions
  • 21. Preparation and functional analysis of HMGN proteins
  • 22. Methods for visualizing chromatin dynamics in living yeast
  • 23. Direct visualization of transcription factor-induced chromatin remodeling and cofactor recruitment in vivo
  • 24. Measuring histone and polymerase dynamics in living cells
  • 25. Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopy
  • 26. Quantification of protein-protein and protein-DNA interactions in vivo, using fluorescence recovery after photobleaching
  • 27. Fluorescence recovery after photobleaching (FRAP) methods for visualizing protein dynamics in living mammalian cell nuclei
  • 28. Correlative light and electron spectroscopic imaging of chromatin in situ
  • 29. RNA fluorscence in situ hybridization tagging and recovery of associated proteins to analyze in vivo chromatin interactions
  • 30. 3C technology: analyzing the spatial organization of genomic loci in vivo / Erik Splinter, Frank Grosveld, and Wouter de Laat.

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