Cell Biological Applications of Confocal Microscopy /

• Άλλοι συγγραφείς: Matsumoto, Brian (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego : Academic Press, 2002.
• Έκδοση: Second edition.
• Σειρά: Methods in cell biology ; v. 70.
• Θέματα: Confocal microscopy.; Microscopy.; Microscopie confocale.; Microscopie.; Microscopie confocale > Méthodes.; NATURE > Reference.; SCIENCE > Life Sciences > General.; SCIENCE > Life Sciences > Biology.; Celbiologie.; Confocale microscopie.; Biologia celular.; Microscopy, Confocal > methods.; Cytological Techniques.; Microscopy, Fluorescence > methods.; Electronic books.

 This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Key Features* Includes detailed protocols accessible to new user. 

 Introduction to confocal microscopy -- Direct-view high-speed confocal scanner : the CSU-10 -- Introduction to multiphoton excitation imaging for the biological sciences -- Confocal microscopy : important considerations for accurate imaging -- Multicolor laser scanning confocal immunofluorescence microscopy : practical application and limitations -- Practical aspects of objective lens selection for confocal and multiphoton digital imaging techniques -- Resolution of subcellular detail in thick tissue sections : immunohistochemical preparation and fluorescence confocal microscopy -- Confocal fluorescence microscopy for tetrahymena thermophila -- Confocal imaging for Drosophila embryos -- Confocal fluorescence microscopy of the cytoskeleton of amphibian oocytes and embryos -- Confocal fluorescence microscopy measurements of pH and calcium in living cells -- Confocal and nonlinear optical imaging of potentiometric dyes -- Measurement and intracellular Ca²+ concentration -- Running and setting up a confocal microscope core facility.