Methods in Cell-Matrix Adhesion /

• Άλλοι συγγραφείς: Adams, Josephine C. (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego : Academic Press, 2002.
• Σειρά: Methods in cell biology ; v. 69.
• Θέματα: Cell adhesion.; Extracellular matrix.; Cell Adhesion.; Cell-Matrix Junctions.; Extracellular Matrix.; Cellules > Adhésivité; Substance fondamentale (Histologie); SCIENCE > Life Sciences > Cell Biology.; Laboratory Manuals.; Electronic books.

 Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual backgrou. 

 pt. 1. Preface and perspectives on cell-matrix adhesion -- Cell-matrix interactions: the view from the outside / Paul Bornstein -- Matrix and meaning / Martin A. Schwartz -- Cell-matrix adhesion research and the development of biotherapeutics / Roy R. Lobb -- pt. 2. Matrix methodologies -- Detection and purification of instructive extracellular matrix components with monoclonal antibody technologies / Susana G. Gil -- Isolation and purification of proteoglycans / John M. Whitelock -- Expression of recombinant matrix components using baculoviruses / Deane F. Mosher -- Heparan sulfate -- growth factor interactions / Alan C. Rapraeger -- Analysis of basement membrane self-assembly and cellular interactions with native and recombinant glycoproteins / Peter D. Yurchenco -- Preparation and analysis of synthetic multicomponent extracellular matrix / Kim S. Midwood -- Analysis of matrix dynamics by atomic force microscopy / Helen G. Hansma -- Analysis of matrix degradation / H.G. Munshi -- pt. 3. Adhesion receptor methodologies -- Expression cloning strategies for the identification of adhesion molecules / Joe W. Ramos -- Purification of integrins and characterization of integrin-associated proteins / Johannes A. Eble -- Methods for analysis of the integrin ligand binding event / Jeffrey W. Smith -- Intracellular coupling of adhesion receptors: molecular proximity measurements / Maddy Parsons -- pt. 4. Functional applications of cell-matrix adhesion in molecular cell biology -- Functional analysis of cell adhesion: quantiation of cell-matrix attachment / Steven K. Akiyama -- Measurements of glycosaminoglycan-based cell interactions / J. Kevin Langford -- Applications of adhesion molecule gene knockout cell lines / Jordan A. Kreidberg -- Flexible polyacrylamide substrata for the analysis of mechanical interactions at cell-substratum adhesions / Karen A. Beningo -- Cell migration in slice cultures / Donna J. Webb -- Application of cell adhesion to study signaling networks / Cindy K. Miranti -- Use of micropatterned adhesive surfaces for control of cell behavior / Philip LeDuc -- Adenoviral-mediated gene transfer in two-dimensional and three-dimensional cultures of mammary epithelial cells / Harriet Watkin.