RNA editing /

• Άλλοι συγγραφείς: Gott, Jonatha M.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego, Calif. : Academic Press/Elsevier, ©2007.
• Σειρά: Methods in enzymology ; v. 424.
• Θέματα: RNA editing.; SCIENCE > Life Sciences > Molecular Biology.; RNA Editing [MESH]; RNA [MESH]; Electronic books.

 RNA processing plays a critical role in realizing the full potential of a given genome. One means of achieving protein diversity is through RNA editing. A diverse array of editing events has been characterized, affecting gene expression in organisms from viruses and single cell parasites to humans and plants. The variety of editing mechanisms has required the development of many different experimental approaches, many of which are likely to be broadly applicable, particularly given the interplay between editing and other cellular processes, including transcription, splicing, and RNA silencing. This volume not only covers most of the principal methods employed in the field, but also offers innovative solutions to the significant challenges posed by these experimental systems. * Presents newly developed methods * Covers topics ranging from biochemistry to bioinformatics * Includes innovative solutions to potential problems. 

 Isolation and compositional analysis of trypanosomatid editosomes / A.K. Panigrahi [and others] -- Uridine insertion/deletion editing activities / J. Carnes [and others] -- RNA editing uridylyltransferases of trypanosomatids / R. Aphasizhev [and others] -- Isolation of RNA binding proteins involved in insertion/deletion editing / M. Pelletier [and others] -- RNA-protein interactions in assembled editing complexes in trypanosomes / J. Cruz-Reyes [and others] -- Strategies of kinetoplastid cryptogene discovery and analysis / D.A. Maslov [and others] -- RNA editing in Physarum mitochondria: Assays and biochemical approaches / E.M. Byrne [and others] -- Computational approaches to insertional RNA editing / R. Bundschuh [and others] -- Evolution of RNA editing sites in the mitochondrial small subunit rRNA of the Myxomycota / U. Krishnan [and others] -- Analysis of 5'- or 3'-terminal trna editing: Mitochondrial 5' tRNA editing in Acanthamoeba castellanii as the exemplar / A.J. Lohan [and others] -- Comparative genomic and bioinformatic approaches for the identification of new adenosine-to-inosine substrates / J. Sixsmith [and others] -- Genetic approaches to studying adenosine-to-inosine RNA editing / J.E.C. Jepson [and others] -- A method for finding sites of selective adenosine deamination / J. Ohlson [and others] -- Purification and assay of ADAR activity / L.P. Keegan [and others] -- Large-scale overexpression and purification of ADARs from Saccharomyces cerevisiae for biophysical and biochemical studies / M.R. Macbeth [and others] -- Mouse models to elucidate the functional roles of adenosine-to-inosine editing / E.Y. Rula [and others] -- Probing adenosine-to-inosine editing reactions using RNA-containing nucleoside analogs / O. Maydanovych [and others] -- Measuring editing activity and identifying cytidine-to-uridine mrna editing factors in cells and biochemical isolates / H.C. Smith [and others] -- Mouse models as tools to explore cytidine-to-uridine RNA editing / S. Cho [and others] -- RNA editing in plant mitochondria: Assays and biochemical approaches / M. Takenaka [and others] -- Assay of editing of exogenous RNAs in chloroplast extracts of Arabidopsis, maize, pea, and tobacco / M.L. Hayes [and others] -- In organello gene expression and RNA editing studies by electroporation-mediated transformation of isolated plant mitochondria / J. Farre [and others] -- Transformation of the plastid genome to study RNA editing / K.A. Lutz [and others].