Lipidomics and bioactive lipids : lipids and cell signaling /

• Other Authors: Brown, H. Alex (Hugh Alex), 1960-2017
• Format: eBook
• Language: English
• Published: Amsterdam, Netherlands : San Diego : Elsevier ; Academic Press, ©2007.
• Series: Methods in enzymology ; v. 434.
• Subjects: Lipids > Analysis.; Lipids > Spectra.; Mass spectrometry.; SCIENCE > Chemistry > Organic.; Lipiden.; Signalen.; Bioactivering.; Biologische activiteit.; Fosfolipasen.; Fosfatidylinositol.; Enzymologie; Lipids > analysis [MESH]; Lipids [MESH]; Mass Spectrometry [MESH]; Electronic books.

 This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae. 

 5050 Phospholipase A₁ assays using a radiolabeled substrate and mass spectrometry / Rei Morikawa [and others] -- Real-time cell assays of phospholipase A₂s using fluorogenic phospholipids / Debasis Manna and Wonhwa Cho -- Analysis and pharmacological targeting of phospholipase C [beta] interactions with G proteins / David M. Lehmann, Chujun Yuan, and Alan V. Smrcka -- Biochemical analysis of phospholipase D / H. Alex Brown [and others] -- Measurement of autotaxin/lysophospholipase D activity / Andrew J. Morris and Susan S. Smyth -- Platelet-activating factor / John S. Owen, Michael J. Thomas and Robert L. Wykle -- Quantitative measurement of phosphatidylinositol 3,4,5-trisphosphate / Herve Guillou, Len R. Stephens and Phillip T. Hawkins -- Measuring phosphorylated Akt and other phosphoinositide ₃-kinase-regulated phosphoproteins in primary lymphocytes / Amber C. Donahue, Michael G. Kharas and David A. Fruman -- Regulation of phosphatidylinositol ₄-phosphate ₅-kinase activity by partner proteins / Yasunori Kanaho [and others] -- Biochemical analysis of inositol phosphate kinases / James C. Otto [and others] -- Analysis of phosphoinositides and their aqueous metabolites / Christopher P. Berrie [and others] -- Combination of C₁-- sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism / Stefka Spassieva [and others] -- Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo / Michael Maceyka, Sheldon Milstein and Sarah Spiegel -- A rapid and sensitive method to measure secretion of sphingosine-1-phosphate / Poulami Mitra [and others] -- Ceramide kinase and ceramide-1-phosphate / Dayanjan S. Wijesinghe [and others] -- Measurement of mammalian diacylglycerol kinase activity in vitro and in cells / Richard M. Epand and Matthew K. Topham -- Lipid phosphate phosphatases from saccharomyces cerevisiae / George M. Carman and Wen-I Wu.