RNA modification /

• Άλλοι συγγραφείς: Gott, Jonatha M.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego, Calif. : Academic Press/Elsevier, ©2007.
• Σειρά: Methods in enzymology ; v. 425.
• Θέματα: RNA editing.; SCIENCE > Life Sciences > Molecular Biology.; RNA Editing [MESH]; RNA [MESH]; Electronic books.

 The presence of modified nucleotides in cellular RNAs has been known for decades and over 100 distinct RNA modifications have been characterized to date. While the exact role of many of these modifications is still unclear, many are highly conserved across evolution and most contribute to the overall fitness of the organism. In recent years, new methods and bioinformatics approaches have been developed for the dissection of modification pathways and functions. These methods intersect a number of related fields, ranging from RNA processing to comparative genomics and systems biology. In addition, many of the techniques described in this volume have broad applicability, particularly in regards to the isolation, characterization, and reconstitution of ribonucleoprotein complexes, expanding the experimental repertoire available to all RNA researchers. 

 Identifying modifications in RNA by MALDI mass spectrometry /   S. Douthwaite and F. Kirpekar --   Identification of modified residues in RNAs by reverse transcription-based methods /   Y. Motorin [and others] --   Detection of enzymatic activity of transfer RNA modification enzymes using radiolabeled tRNA substrates /   H. Grosjean [and others] --   In vitro detection of the enzymatic activity of folate-dependent tRNA (uracil-54,-c5)-methyltransferase: Evolutionary implications /   J. Urbonavicius [and others] --   Probing the intermediacy of covalent RNA enzyme complexes in RNA modification enzymes /   S.M. Chervin [and others] --   Identification and characterization of modification enzymes by biochemical analysis of the proteome /   J.E. Jackman [and others] --   Identification of genes encoding tRNA modification enzymes by comparative genomics /   V. de Crecy-Lagard --   Identification and characterization of archaeal and fungal tRNA methyltransferases /   D.E. Graham and G. Kramer --   Mass spectrometric identification and characterization of RNA-modifying enzymes /   T. Suzuki [and others] --   Chaplet column chromatography: Isolation of a large set of individual RNAs in a single step /   T. Suzuki and T. Suzuki --   Biochemical purification of box H/ACA RNPs involved in pseudouridylation /   J. Karijolich [and others] --   In vitro reconstitution and affinity purification of catalytically active archaeal box C/D sRNP complexes /   K. Gagnon [and others] --   Identifying effects of snoRNA-guided modifications on the synthesis and function of the yeast ribosome /   W.A. Decatur [and others] --   The U1 snRNA hairpin II as a RNA affinity tag for selecting snoRNP complexes /   D. Piekna-Przybylska [and others] --   A dedicated computational approach for the identification of archaeal H/ACA sRNAs /   S. Muller [and others] --   Reconstitution of archaeal H/ACA sRNPs and test of their activity / B. Charpentier [and others].