Gene transfer and expression in mammalian cells /

• Άλλοι συγγραφείς: Makrides, Savvas C.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam : Elsevier Science, 2003.
• Σειρά: New comprehensive biochemistry ; v. 38.
• Θέματα: Genetic engineering.; Genetic transformation.; Gene expression.; Gene Expression > physiology.; Genetic Engineering > methods.; Genetic Vectors.; Transformation, Genetic > physiology.; Viruses.; Expression génique.; Electronic books.

 The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors. This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field. 

 Why choose mammalian cells for protein production? / Savvas C. Makrides, Holly L. Prentice -- Vectors for gene expression in mammalian cells / Savvas C. Makrides -- Virus-based vectors for gene expression in mammalian cells. Herpes simplex virus / Edward A Burton, Qing Bai, William F. Goins, David J. Fink, Joseph C. Glorioso -- Virus-based vectors for gene expression in mammalian cells. Epstein-Barr virus / Gregory Kennedy, Bill Sugden -- Virus-based vectors for gene expression in mammalian cells. SV40 / David S. Strayer, Pierre Cordelier, Julien Landré, Alexei Matskevitch, Hayley J. McKee, Carmen N. Nichols, Martyn K. White, Marlene S. Strayer -- Virus-based vectors for gene expression in mammalian cells. Adeno-associated virus / Xiao Xiao -- Virus-based vectors for gene expression in mammalian cells. Adenovirus / Denis Bourbeau, Yué Zeng, Bernard Massie -- Virus-based vectors for gene expression in mammalian cells. Vaccinia virus / Miles W. Carroll, Gerald R. Kovacs -- Virus-based vectors for gene expression in mammalian cells. Baculovirus / J. Patrick Condreay, Thomas A. Kost -- Virus-based vectors for gene expression in mammalian cells. Coronavirus / Luis Enjuanes, Fernando Almazán, Javier Ortego -- Virus-based vectors for gene expression in mammalian cells. Poliovirus / Shane Crotty, Raul Andino -- Virus-based vectors for gene expression in mammalian cells. Sindbis virus / Henry V. Huang, Sondra Schlesinger -- Virus-based vectors for gene expression in mammalian cells. Semliki Forest virus / Kenneth Lundstrom -- Virus-based vectors for gene expression in mammalian cells. Retrovirus / Cristina Parolin, Giorgio Palù -- Virus-based vectors for gene expression in mammalian cells. Lentiviruses / Mehdi Gasmi, Flossie Wong-Staal -- Methods for DNA introduction into mammalian cells / Pamela A. Norton, Catherine J. Pachuk -- Lipid reagents for DNA transfer into mammalian cells / Christophe Masson, Virginie Escriou, Michel Bessodes, Daniel Scherman -- Reporter genes for monitoring gene expression in mammalian cells / Jawed Alam, Julia L Cook -- Gene transfer and gene amplification in mammalian cells / Florian M. Wurm, Martin Jordan -- Co-transfer of multiple plasmids/viruses as an attractive method to introduce several genes in mammalian cells / Martin Jordan, Florian M. Wurm -- Optimization of plasmid backbone for gene expression in mammalian cells / Pascal Bigey, Marie Carrière, Daniel Scherman -- Use of scaffold/matrix-attachment regions for protein production / Pierre-Alain Girod, Nicolas Mermod -- Chromatin insulators and position effects / David W. Emery, Mari Aker, George Stamatoyannopoulos -- Locus control regions / Xiangdong Fang, Kenneth R. Peterson, Qiliang Li, George Stamatoyannopoulos -- Protein synthesis, folding, modification, and secretion in mammalian cells / M. Stacey Arnold, Randal J. Kaufman -- Pathways and functions of mammalian protein glycosylation / Dale A. Cumming -- Metabolic engineering of mammalian cells for higher protein yield / Hitto Kaufmann, Martin Fussenegger -- Translational regulation in mammalian cells / Marilyn Kozak -- Pathways of mammalian messenger RNA degradation / Angela Inácio, Stephen A. Liebhaber -- Pathways of mammalian protein degradation / William A. Dunn Jr. -- Stabilization of proteasomal substrates by viral repeats / Nico P. Dantuma, Maria G. Masucci -- Architecture and utilization of highly expressed genomic sites / Jürgen Bode, Sandra Götze, Ellen Ernst, Yves Hüsemann, Alexandra Baer, Jost Seibler, Christian Mielke -- Intracellular targeting of antibodies in mammalian cells / Quan Zhu, Wayne A. Marasco -- Inducible gene expression in mammalian cells / Wilfried Weber, Martin Fussenegger -- Protein production by large-scale mammalian cell culture / Liangzhi Xie, Weichang Zhou, David Robinson -- Protein production in transgenic animals / Yann Echelard, Harry M. Meade -- Strategies for the purification of recombinant proteins / Steven L. Giardina.