Intermediate Filament Cytoskeleton /

• Άλλοι συγγραφείς: Omary, M. Bishr (Επιμελητής έκδοσης), Coulombe, Pierre A. (Επιμελητής έκδοσης)
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam ; Boston : Elsevier Academic Press, 2004.
• Σειρά: Methods in cell biology ; v. 78.
• Θέματα: Cytoplasmic filaments.; Cytoskeletal proteins.; Intermediate Filament Proteins > physiology.; Filaments cytoplasmiques.; Cytoskelet.; Laboratory Manuals.; Electronic books.

Intermediate filaments are a large family of proteins that are the cytoskeletal elements involved in a number of skin, liver, neuromuscular, cardiac, eye and hair diseases. Intermediate filament genes are regulated in a tissue-and cell type-specific manner and their polymerized protein products prot...

Πίνακας περιεχομένων:

• Isolation, characterization, and in vitro assembly of intermediate filaments
• Intermediate filament protein structure determination
• Intracellular microrheology as a tool for the measurement of the local mechanical properties of live cells
• Developing mouse models to study intermediate filaments in apoptosis
• Uncovering the roles of intermediate filaments in apoptosis
• Molecular genetics methods for human intermediate filament diseases
• Intermediate filaments and multiparameter flow cytometry for the study of solid tumors
• Intermediate filament protein inclusions
• Stress models for the study of intermediate filament function
• Regulation of intermediate filament gene expression
• Fluorescence-based methods for studying intermediate filaments
• Imaging of keratin dynamics during the cell cycle and in response to phosphatase inhibition
• Approaches to study phosphorylation filament proteins using site-specific and phosphorylation state-specific antibodies
• Approaches to study posttranslational regulation of intermediate filament proteins
• Hair keratins and hair follicle-specific epithelial keratins
• Skin : an ideal model system to study keratin genes and proteins
• Studying simple epithelial keratins in cells and tissues
• Muscle intermediate filament proteins
• [35S] methionine metabolic labeling to study axonal transport of neuronal intermediate filament proteins in vivo
• Lamins
• The intermediate filament systems in the eye lens
• Fish keratins
• Performing functional studies of xenopus laevis intermediate filament proteins through injection og macromolecules into early embryos
• Intermediate filaments in caenorhabditis elegans
• Plectin
• In vitro methods for investigating desmoplakin-intermediate filament interactions and their role in adhesive strength
• Studying cytolinker
• Keratin bundling proteins
• Lamin-associated proteins.