Adenylyl cyclase, G proteins, and guanylyl cyclase /

• Other Authors: Johnson, Roger A., Corbin, Jackie D.
• Format: eBook
• Language: English
• Published: San Diego : Academic Press, ©1991.
• Series: Methods in enzymology ; v. 195.
• Subjects: Adenylate cyclase.; G proteins.; Adenylyl Cyclases > isolation & purification.; GTP-Binding Proteins > isolation & purification.; Guanylyl Imidodiphosphate > isolation & purification.; Electronic books.
• Physical Description: 1 online resource (xxx, 512 pages) : illustrations.
• Bibliography: Includes bibliographical references and indexes.
• ISBN: 0121820963; 9780121820961
• ISSN: 0076-6879 ;

This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. Key Features * Adenylyl cyclase: determination of activity * Preparation of materials useful in purification of components of hormonally responsive cyclase systems * Purification guanine nucleotide-dependent regulatory proteins * Purification and characterization of g proteins: labeling and quantitating of G proteins * Reconstitution guanylyl cyclase: assay * Preparation of materials used for study of the cyclase * Purification * Regulation.