Adenylyl cyclase, G proteins, and guanylyl cyclase /

• Άλλοι συγγραφείς: Johnson, Roger A., Corbin, Jackie D.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego : Academic Press, ©1991.
• Σειρά: Methods in enzymology ; v. 195.
• Θέματα: Adenylate cyclase.; G proteins.; Adenylyl Cyclases > isolation & purification.; GTP-Binding Proteins > isolation & purification.; Guanylyl Imidodiphosphate > isolation & purification.; Electronic books.

 This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. Key Features * Adenylyl cyclase: determination of activity * Preparation of materials useful in purification of components of hormonally responsive cyclase systems * Purification guanine nucleotide-dependent regulatory proteins * Purification and characterization of g proteins: labeling and quantitating of G proteins * Reconstitution guanylyl cyclase: assay * Preparation of materials used for study of the cyclase * Purification * Regulation. 

 t Assay of adenylyl cyclase catalytic activity / r Roger A. Johnson [and others] -- t Cellular responsiveness to hormones and neutrotransmitters: Conversion of [3H]Adenine to [3H]cAMP in cell monolayers, cell suspensions, and tissue slices / r Yoram Salomon, Roger A. Johnson and Jackie D. Corbin -- t Preparation of [alpha]-32P-Labeled nucleoside triphosphates, nicotinamide adenine dinucleotide, and cyclic nucleotides for use in determining adenylyl and guanylyl cyclases and cyclic nucleotide phosphodiesterase / r Timothy F. Walseth [and others] -- t Synthesis of forskolin-agarose affinity matrices / r Thomas Pfeuffer, Roger A. Johnson and Jackie D. Corbin -- t High-affinity binding sites for [3H]forskolin / r Antonio Laurenza [and others] -- t Purification and characterization of calmodulin-sensitive adenylyl cyclase from bovine brain / r Ping Wang [and others] -- t Purification of adenylyl cyclase from heart and brain / r Elke Pfeuffer [and others] -- t Calmodulin-mediated adenylyl cyclase from equine sperm / r William A. Toscano [and others] -- t Isolation of polyclonal antibodies against bovine brain calmodulin-sensitive adenylyl cyclases / r Gary B. Rosenberg [and others] -- t Characteristics and use of monoclonal antibodies to various forms of adenylyl cyclase / r Stefan Mollner [and others] -- t Purification of soluble form of adenylyl cyclase from testes / r Theodor Braun, Roger A. Johnson and Jackie D. Corbin -- t Purification and assay of cell-invasive form of calmodulin-sensitive adenylyl cyclase from Bordetella pertussis / r H. Robert Masure [and others] -- t Purification and characterization of adenylyl cyclase from Bacillus anthracis / r Stephen H. Leppla, Roger A. Johnson and Jackie D. Corbin -- t Preparation and application of GTP-agarose matrices / r Elke Pfeuffer [and others] -- t Purification of G proteins / r Juan Codina [and others] -- t Preparation of guanine nucleotide-free G proteins / r Kenneth M. Ferguson [and others] -- t Purification of recombinant Gs[alpha] / r Michael P. Graziano [and others] -- t Purification of recombinant G1[alpha] and Go[alpha] proteins from Escherichia coli / r Maurine E. Linder [and others] -- t Synthetic peptide antisera with determined specificity for G protein [alpha] or [beta] subunits / r Susanne M. Mumby [and others] -- t Quantitation and purification of ADP-ribosylation factor / r Richard A. Kahn, Roger A. Johnson and Jackie D. Corbin -- t Soluble guanine nucleotide-dependent ADP-ribosylation factors in activation of adenylyl cyclase by cholera toxin / r Joel Moss [and others] -- t ADP-ribosylation of G proteins with pertussis toxin / r Gregory S. Kopf [and others] -- t Cholera toxin-catalyzed [32P]ADP-ribosylation of proteins / r D. Michael Gill [and others] -- t Photoaffinity labeling of GTP-binding proteins / r Rolf Thomas [and others].