Adenylyl cyclase, G proteins, and guanylyl cyclase /
This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. Key Features * Adenylyl cyclase: determination of activity * Preparation of materials useful in purification of...
| Άλλοι συγγραφείς: | , |
|---|---|
| Μορφή: | Ηλ. βιβλίο |
| Γλώσσα: | English |
| Έκδοση: |
San Diego :
Academic Press,
©1991.
|
| Σειρά: | Methods in enzymology ;
v. 195. |
| Θέματα: | |
| Διαθέσιμο Online: | Full Text via HEAL-Link |
Πίνακας περιεχομένων:
- t Assay of adenylyl cyclase catalytic activity / r Roger A. Johnson [and others]
- t Cellular responsiveness to hormones and neutrotransmitters: Conversion of [3H]Adenine to [3H]cAMP in cell monolayers, cell suspensions, and tissue slices / r Yoram Salomon, Roger A. Johnson and Jackie D. Corbin
- t Preparation of [alpha]-32P-Labeled nucleoside triphosphates, nicotinamide adenine dinucleotide, and cyclic nucleotides for use in determining adenylyl and guanylyl cyclases and cyclic nucleotide phosphodiesterase / r Timothy F. Walseth [and others]
- t Synthesis of forskolin-agarose affinity matrices / r Thomas Pfeuffer, Roger A. Johnson and Jackie D. Corbin
- t High-affinity binding sites for [3H]forskolin / r Antonio Laurenza [and others]
- t Purification and characterization of calmodulin-sensitive adenylyl cyclase from bovine brain / r Ping Wang [and others]
- t Purification of adenylyl cyclase from heart and brain / r Elke Pfeuffer [and others]
- t Calmodulin-mediated adenylyl cyclase from equine sperm / r William A. Toscano [and others]
- t Isolation of polyclonal antibodies against bovine brain calmodulin-sensitive adenylyl cyclases / r Gary B. Rosenberg [and others]
- t Characteristics and use of monoclonal antibodies to various forms of adenylyl cyclase / r Stefan Mollner [and others]
- t Purification of soluble form of adenylyl cyclase from testes / r Theodor Braun, Roger A. Johnson and Jackie D. Corbin
- t Purification and assay of cell-invasive form of calmodulin-sensitive adenylyl cyclase from Bordetella pertussis / r H. Robert Masure [and others]
- t Purification and characterization of adenylyl cyclase from Bacillus anthracis / r Stephen H. Leppla, Roger A. Johnson and Jackie D. Corbin
- t Preparation and application of GTP-agarose matrices / r Elke Pfeuffer [and others]
- t Purification of G proteins / r Juan Codina [and others]
- t Preparation of guanine nucleotide-free G proteins / r Kenneth M. Ferguson [and others]
- t Purification of recombinant Gs[alpha] / r Michael P. Graziano [and others]
- t Purification of recombinant G1[alpha] and Go[alpha] proteins from Escherichia coli / r Maurine E. Linder [and others]
- t Synthetic peptide antisera with determined specificity for G protein [alpha] or [beta] subunits / r Susanne M. Mumby [and others]
- t Quantitation and purification of ADP-ribosylation factor / r Richard A. Kahn, Roger A. Johnson and Jackie D. Corbin
- t Soluble guanine nucleotide-dependent ADP-ribosylation factors in activation of adenylyl cyclase by cholera toxin / r Joel Moss [and others]
- t ADP-ribosylation of G proteins with pertussis toxin / r Gregory S. Kopf [and others]
- t Cholera toxin-catalyzed [32P]ADP-ribosylation of proteins / r D. Michael Gill [and others]
- t Photoaffinity labeling of GTP-binding proteins / r Rolf Thomas [and others].
- t Identification of receptor-activated G proteins with photoreactive GTP analog, [[alpha]-32P]GTP azidoanilide / r Stefan Offermanns [and others]
- t Quantitative immunoblotting of G-protein subunits / r Donna J. Carty [and others]
- t Assay of g-protein [beta][gamma]-subunit complex by catalytic support of ADP-ribosylation of Go[alpha] / r Patrick J. Casey [and others]
- t Tryptophan fluorescence of G proteins: Analysis of guanine nucleotide binding and hydrolysis / r Tsutomu Higashijima [and others]
- t Reconstitution of receptors and G proteins in phospholipid vesicles / r Richard A. Cerione [and others]
- t Assay of guanylyl cyclase catalytic activity / r Steven E. Domino [and others]
- t Preparative polyacrylamide gel electrophoresis apparatus for purification of guanylyl cyclase / r Alexander Mul̈sch [and others]
- t Detergent interactions and solubilization techniques for membrane guanylyl cyclase / r Arnold A. White [and others]
- t Purification of membrane form of guanylyl cyclase / r Chodavarapu S. Ramarao [and others]
- t Purification of heme-containing soluble guanylyl cyclase / r Alexander Mul̈sch [and others]
- t Preparation of soluble guanylyl cyclase from bovine lung by immunoaffinity chromatography / r Peter Humbert [and others]
- t Immunoaffinity purification of soluble guanylyl cyclase / r Scott A. Waldman [and others]
- t Copurification of atrial natriuretic peptide receptor and particulate guanylyl cyclase / r Scott A. Waldman [and others]
- t Copurification of atrial natriuretic factor receptors and guanylyl cyclase from adrenal cortex / r Tadashi Inagami [and others]
- t Molecular cloning of membrane forms of guanylyl cyclase / r Sujay Singh [and others]
- t Radiation-inactivation analysis of multidomain proteins: The case of particulate guanylyl cyclase / r Michel Potier [and others]
- t Identification of atrial natriuretic peptide receptors in cultured cells / r Dale C. Leitman [and others]
- t Evaluating atrial natriuretic peptide-induced cGMP production by particulate guanylyl cyclase stimulation in Vitro and in Vivo / r Pavel Hamet [and others]
- t Phosphorylation and dephosphorylation of sea urchin sperm cell guanylyl cyclase / r J. Kelley Bentley, Roger A. Johnson and Jackie D. Corbin
- t Calcium-regulated guanylyl cyclases from Paramecium and Tetrahymena / r Joachim E. Schultz [and others].
