Protein engineering /
This MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies.
| Άλλοι συγγραφείς: | , |
|---|---|
| Μορφή: | Ηλ. βιβλίο |
| Γλώσσα: | English |
| Έκδοση: |
Amsterdam ; Boston :
Elsevier Academic Press,
©2004.
|
| Σειρά: | Methods in enzymology ;
v. 388. |
| Θέματα: | |
| Διαθέσιμο Online: | Full Text via HEAL-Link |
Πίνακας περιεχομένων:
- Section I. Methodology
- Gene site saturation mutagenesis : a comprehensive mutagenesis approach
- Single-stranded DNA family shuffling
- Module shuffling
- SCHEMA-guided protein recombination
- Staggered extension process in vitro DNA recombination
- Using incremental truncation to create libraries of hybrid enzymes
- Random multirecombinant polymerase chain reaction
- Gene library synthesis by structure-based combinatorial protein engineering
- New enzymes from combinatorial library modules
- Construction of protein fragment complementation libraries using incremental truncation
- Section II. Applications: optimization and screening
- GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform
- High throughput microplate screens for directed protein evolution
- Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities
- Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis
- Screening for oxidative resistance
- Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes
- Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system
- Section III. Applications: directed evolution of enzymatic function
- Directed evolution of lipases and esterases
- Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium
- Directed evolution of aldolases
- Changing the enantioselectivity of enzymes by directed evolution
- Control of stereoselectivity in phosphotriesterase
- Section IV. Applications: evolution of biosynthetic pathways
- Manipulation and analysis of polyketide synthases
- Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases
- Engineering carotenoid biosynthetic pathways
- Section V. Devices, antibodies, and vaccines
- Directed evolution of bacteriorhodopsin for device applications
- Engineering antibody affinity by yeast surface display
- A conditionally replicating virus as a novel approach toward an HIV vaccine.
