Initiation and termination of cyclic nucleotide action. Volume 159 /

• Άλλοι συγγραφείς: Corbin, Jackie D., Johnson, Roger A.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego : Academic Press, ©1988.
• Σειρά: Methods in enzymology ; volume 159.
• Θέματα: Cyclic nucleotides.; Phosphatases.; Nucléotides cycliques.; Nucleotides, Cyclic > isolation & purification.; Nucleotides, Cyclic > metabolism.; Phosphoric Diester Hydrolases > isolation & purification.; Acido nucleico; DNA; Nucelotodeo; Nucleic acid; Nucleotide; Electronic books.

 Novel approaches for the study of phosphodiesterases and phosphoprotein phosphatases, as well as those of nonmammalian cyclic nucleotide receptors, are emphasized for the first time in the Methods of Enzymology series. New methods for the study of protein kinases are also presented. 

 t Role of cAMP in cyclic cascade regulation / r Emily Shacter [and others] -- t Theoretical considerations of the regulatory steps in the cAMP cascade system / r Steṕhane Swillens [and others] -- t Yeast mutants altered in the cAMP cascade system / r Tatsuo Ishikawa [and others] -- t Improvements in the automated radioimmunoassay for CAMP or cGMP / r Gary Brooker, Jackie D. Corbin and Roger A. Johnson -- t cAMP turnover in intact cells / r R. Barber [and others] -- t Monitoring cyclic nucleotide metabolism in intact cells by 18O labeling / r Timothy F. Walseth [and others] -- t Purification and assay of cAMP, cGMP, and cyclic nucleotide analogs in cells treated with cyclic nucleotide analogs / r Jackie D. Corbin [and others] -- t cAMP export and its regulation by prostaglandin A1 / r Laurence L. Brunton [and others] -- t Use of antibody- sepharose columns to study hormonal activation of cAMP-dependent protein kinase isozymes / r Roald Ekanger [and others] -- t Selective activation of the cAMP-dependent protein kinase isoenzymes / r S.A. Livesey [and others] -- t Use of synergistic pairs of site-selective cAMP analogs in intact cells / r Stephen J. Beebe [and others] -- t cAMP-dependent protein kinase regulatory subunit phosphorylation in intact cells / r Marc C. Mumby [and others] -- t Ammonium sulfate precipitation assay for the study of cyclic nucleotide binding to proteins / r Stein Ove Døskeland [and others] -- t cGMP-dependent protein kinase activation in intact tissues / r Ronald R. Fiscus [and others] -- t cAMP analog antagonists of cAMP action / r Lynne H. Parker Botelho [and others] -- t Peptide inhibitors of CAMP-dependent protein kinase / r Bruce E. Kemp [and others] -- t Type II cAMP-dependent protein kinase regulatory subunit-binding proteins / r Suzanne M. Lohmann [and others] -- t Use of digitonin-permeabilized adipocytes for cAMP studies / r Robert A. Mooney, Jackie D. Corbin and Roger A. Johnson -- t Purification of cAMP-free and cAMP-bound forms of bovine heart cAMP-dependent protein kinase holoenzyme / r Charles E. Cobb [and others] -- t Preparation of regulatory subunits from bovine heart cAMP-dependent protein kinase by a nondenaturing method / r Mark Seville [and others] -- t Purification of Drosophila cAMP-dependent protein kinase / r John L. Foster [and others] -- t Using immunocolloidal gold electron microscopy to investigate cAMP-dependent protein kinase cellular compartmentalization / r Richard A. Jungmann [and others].