Guide to protein purification /
Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features *...
| Άλλοι συγγραφείς: | |
|---|---|
| Μορφή: | Ηλ. βιβλίο |
| Γλώσσα: | English |
| Έκδοση: |
San Diego :
Academic Press,
©1990.
|
| Σειρά: | Methods in enzymology ;
v. 182. |
| Θέματα: | |
| Διαθέσιμο Online: | Full Text via HEAL-Link |
Πίνακας περιεχομένων:
- Why purify enzymes / Arthur Kornberg
- Strategies and considerations for protein purifications / Stuart Linn
- Setting up a laboratory / Murray P. Deutscher
- Buffers: Principles and practice / Vincent S. Stoll, John S. Blanchard
- Measurement of enzyme activity / Edward F. Rossomando
- Quantitation of protein / Christa M. Stoscheck
- Concentration of proteins and removal of solutes / Thomas Pohl
- Maintaining protein stability / Murray P. Deutscher
- Overproduction of proteins in Escherichia coli: Vectors, hosts, and strategies / Asis Das
- Overexpression of proteins in eukaryotes / Margaret K. Bradley
- Secretion of heterologous proteins in Escherichia coli / I. Barry Holland, Brendan Kenny, Boris Steipe, Andreas Plückthun
- Preparation of extracts from prokaryotes / Millard Cull, Charles S. McHenry
- Preparation of extracts from yeast / S. Michal Jazwinski
- Preparation of Extracts from Plants / Peter Gegenheimer
- Preparation of extracts from higher eukaryotes / John David Dignam
- Isolation of subcellular organelles / Brian Storrie, Edward A Madden
- Preparation of membrane fractions / Juris Ozols
- Detergents: An overview / Judith M. Neugebauer
- Solubilization of native membrane proteins / Leonard M. Hjelmeland
- Solubilization of protein aggregates / Fiona A.O. Marston, Donna L. Hartley
- Removal of detergents from membrane proteins / Leonard M. Hjelmeland
- Precipitation techniques / Sasha Englard, Sam Seifter
- Precipitation of proteins with polyethylene glycol / Kenneth C. Ingham
- Ion-exchange chromatography / Edward F. Rossomando
- Gel filtration / Earle Stellwagen
- Protein chromatography on hydroxyapatite columns / Marina J. Gorbunoff
- Hydrophobic chromatography / Robert M. Kennedy
- Chromatography on immobilized reactive dyes / Earle Stellwagen
- Affinity chromatography: General methods / Steven Ostrove
- Affinity chromatography: Specialized techniques / Steven Ostrove, Shelly Weiss
- Chromatofocusing / Lallan Giri
- High-performance liquid chromatography: Effective protein purification by various chromatographic modes / Roman M. Chicz, Fred E. Regnier
- One-dimensional gel electrophoresis / David E. Garfin
- Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura, Therese M. Timmons
- Isoelectric focusing / David E. Garfin
- Gel-staining techniques / Carl R. Merril
- Elution of protein from gels / Michael G. Harrington
- Purification of membrane proteins / Thomas C. Thomas, Mark G. McNamee
- Purification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg, Stephen C. Francesconi, Chris Civalier, Scott S. Walker
- Purification of glycoproteins / Craig Gerard
- Purification of multienzyme complexes / Paul A. Srere, Christopher K. Mathews
- Determination of purity / David G. Rhodes, Thomas M. Laue
- Determination of size, molecular weight, and presence of subunits / Thomas M. Laue, David G. Rhodes
- Amino acid analysis / Juris Ozols
- Limited N-terminal sequence analysis / Paul Matsudaira
- Peptide mapping / Ralph C. Judd
- Analysis for protein modifications and nonprotein cofactors / Sam Seifter, Sasha Englard
- Protein crystallization / David Ollis, Stephen White
- Preparation of polyclonal antibodies / Bonnie S. Dunbar, Eric D. Schwoebel
- Preparation of monoclonal antibodies / Bonnie S. Dunbar, Sheri M. Skinner
- Protein blotting and immunodetection / Therese M. Timmons, Bonnie S. Dunbar
- Immunoprecipitation of proteins / Gary L. Firestone, Sandra D. Winguth
- Immunoassays / Charles W. Parker
- Radiolabeling of proteins / Charles W. Parker
- Using purified protein to clone its gene / John M. Wozney
- Computer analysis of protein structure / Patrick Argos
- Rethinking your purification procedure / Murray P. Deutscher
- The private life of DNA polymerase I / Arthur Kornberg
- Purification and crystallization of transaldolase from Candida utilis / B.L. Horecker, O. Tsolas
- Discovery of glutamine synthetase cascade / Earl R. Stadtman
- Discovery, resolution, purification, and function of elongation factors / Kivie Moldave.
