Viral polymerases and related proteins /

• Άλλοι συγγραφείς: Kuo, Lawrence C., Olsen, David B., Carroll, Steven S.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego : Academic Press, ©1996.
• Σειρά: Methods in enzymology ; v. 275.
• Θέματα: Transferases > Analysis.; Transferases > Purification.; DNA, Viral > physiology.; DNA-Directed DNA Polymerase > isolation & purification.; DNA-Directed RNA Polymerases > isolation & purification.; Enzymes > Related to > Viruses; Electronic books.

 The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Expression, purification, and characterization * Activity assays * Kinetic and screening * Design and analysis of substrates and inhibitors * Molecular and structural characterizations. 

 Sect. 1. Expression, purification, and characterization -- Purification of viral polymerases: general considerations -- Expression, purification, and characterization of the herpes simplex virus type-1 DNA polymerase -- Assays for poliovirus polymerase, 3Dpol, and authentic RNA replication in HeLa S10 extracts -- RNA-dependent RNA polymerase of hepatitis C virus -- Characterization of coronavirus RNA polymerase gene products -- Expression, purification, and characterization of orthomyxovirus: influenza transcriptase -- Expression, purification, and characterization of rhabdovirus polymerase --Expression and purification of retroviral HIV-1 reverse transcriptase -- Expression, purification, and characterization of DNA polymerases involved in papovavirus replication -- Heterologous expression, purification, and characterization of adenovirus DNA polymerase and preterminal protein -- Expression and characterization of hepadnavirus reverse transcriptases -- Expression, purification, and characterization of vaccinia virus-encoded RNA and poly(A)polymerases -- Viral polymerase-associated 5'(arrow)3' exonucleases: expression, purification, and uses -- Sect. 2. Activity assays: kinetic and screening -- Isotopic assays of viral polymerases and related proteins -- Nonisotopic assays of viral polymerases and related proteins -- Catalytic activities associated with retroviral and viral polymerases -- Approaches to high-volume screening assays of viral polymerases and related proteins -- In situ DNA polymerase and RNase H activity gel assays as applied to hepadnavirus particles -- A system to analyze and identify inhibitors of HIV-1 gene regulation using a defective integrated provirus --Sect. 3. Design and analysis of substrates and inhibitors -- Preparation and use of synthetic oligoribonucleotides as tools for study of viral polymerases -- Use of bacteriophage RNA polymerase in RNA synthesis -- Inhibition of viral polymreases by chain-terminating substrates: a kinetic analysis -- Design of nucleoside analog inhibitors of herpes-virus polymerases -- Development of nonucleoside HIV reverse transcriptase inhibitors -- Analysis of inhibition of retroviral reverse transcriptase -- Novel methods of generating specific oligonucleotide inhibitors of viral polymerases -- Sect. 4. Molecular and structural characterizations -- Analyzing the fidelity of reverse transcription and transcription -- Site-directed mutagenic analysis of viral polymerases and related proteins -- Analysis of resistance mutants of viral polymerases -- Author index -- Subject index.