Vitamins and coenzymes. Part L /

• Άλλοι συγγραφείς: McCormick, Donald B. (Donald Bruce), Suttie, J. W. (John Weston), 1934-, Wagner, Conrad
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego : Academic Press, 1997.
• Σειρά: Methods in enzymology ; v. 282.
• Θέματα: Enzymology > Methods.; Enzymology.; Vitamines.; Coenzymes.; Enzymologie.; Vitamines D.; Vitamine A.; Vitamine E.; Vitamines K.; Vitamins.; Electronic books.; Methods (Music)

 The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * This volume and its companion Volumes 279, 280, and 281 provide * A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes * Details on physical, chemical, and biological properties of vitamins and coenzymes * Chemical and biological syntheses of vitamins, coenzymes, and their analogs * Aspects of transport and metabolism of vitamins and coenzymes. 

 Generation and characterization of cellular retinoic acid-binding proteins from Escherichia coli expression systems -- Generation and characterizing retinoid receptors from Escherichia coli and insect cell expression systems -- Expression and characterization of retinoid receptors in yeast -- Use of in situ hybridization techniques to study embryonic expression of retinoid receptors and binding proteins -- Use of quantitative polymerase chain reaction to study retinoid receptor expression -- Use of quantitative polymerase chain reaction to study cellular retinoic acid-binding protein-II mRNA expression -- Use of transgenic mice to study activation of retinoic acid-responsive promoters -- Use of transgenic mice to eliminate retinoic acid receptor function -- Use of reporter cells to study endogenous retinoid sources -- Preparation of radiolabeled 9-cis- and all-trans-retinoids -- Identification and quantification of retinoic acid and other metabolites from [beta]-Carotene excentric cleavage -- Assessing metabolism of [beta]-Carotene.