Regulators and effectors of small GTPases : Rho family /
The Ras superfamily (>150 human members) encompasses Ras GTPases involved in cell proliferation, Rho GTPases involved in regulating the cytoskeleton, Rab GTPases involved in membrane targeting/fusion and a group of GTPases including Sar1, Arf, Arl and dynamin involved in vesicle budding/fission....
| Άλλοι συγγραφείς: | , , |
|---|---|
| Μορφή: | Ηλ. βιβλίο |
| Γλώσσα: | English |
| Έκδοση: |
Amsterdam ; Boston :
Elsevier : Academic Press,
Ã2006.
|
| Σειρά: | Methods in enzymology ;
v. 406. |
| Θέματα: | |
| Διαθέσιμο Online: | Full Text via HEAL-Link |
Πίνακας περιεχομένων:
- Purification and Biochemical Properties of Rac1, 2, 3 and the Splice Variant Rac1b
- Biochemical Analyses of the Wrch Atypical Rho Family GTPases
- Purification of P-Rex1 from Neutrophils and Nucleotide Exchange Assay
- In Vitro Guanine Nucleotide Exchange Activity of DHR-2/DOCKER/CZH2 Domains
- Biochemical Characterization of the Cool (Cloned-Out-of-Library)/Pix (Pak-Interactive Exchange Factor) Proteins
- GEF and Glucosylation Assays on Liposome-Bound Rac
- Phosphorylation of RhoGDI by p21-activated kinase 1
- Purification of ARAP3 and Characterization of GAP Activities
- Regulation of RhoGAP specificity by Phospholipids and Prenylation
- Purification and Activity of the Rho ADP-ribosylating binary C2/C3 Toxin
- Purification of TAT-C3 Exoenzyme
- Imaging and photobleach correction of Mero-CBD, Sensor of Endogenous Cdc42 Activation
- Cdc42 and PI(4,5)P2-induced actin Assembly in Xenopus Egg Extracts
- In vitro reconstitution of Cdc42-mediated actin assembly using purified components
- Biochemical analysis of mammalian formin effects on actin Dynamics
- Formin proteins: purification and measurement of effects on actin assembly
- Purification and kinase assay of PKN
- Purification and enzyme activity of ACK1
- Direct Activation of purified phospholipase C epsilon by RhoA studied in reconstituted phospholipid Vesicles
- Regulation of PLCbeta isoforms by Rac
- Biochemical properties and Inhibitors of (N- )WASP
- The Use of GFP to localize Rho GTPases in living cells
- Analysis of the spatiotemporal activation of Rho GTPases using Raichu probes
- Measurement of activity of Rho GTPases during mitosis
- Inhibition of Rho GTPases by RNA interference
- RNA interference techniques to study epithelial cell adhesion and polarity
- Nucleofection of Primary Neurons
- Dock180-ELMO cooperation in Rac activation
- Rho GTPase activation by cell-cell adhesion
- Activation of Rap1, Cdc42, and Rac by nectin adhesion system
- Analysis of activated GAPs and GEFs in cell lysates
- Degradation of RhoA by Smurf1 ubiquitin ligase
- Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin
- Regulation of superoxide-producing NADPH oxidases in nonphagocytic cells
- Activation of MEKK1 by Rho GTPases
- Activation of the apoptotic JNK pathway through the Rac1-binding scaffold protein POSH
- Quantification of isozyme-specific activation of phospholipase C-beta2 by Rac GTPases and phospholipase C-epsiv by Rho GTPases in an intact cell assay system
- Activiation of Rho and Rac by Wnt/frizzled signaling
- Fluorescent assay of cell-permeable C3 transfease activity
- Use of TIRF microscopy to visualize actin and microtubules in migrating cells
- Inhibition of ROCK by RhoE
- Conditional Regulation of a ROCK-estrogen receptor fusion protein
- Rational design and applications of a Rac GTPase-specific small molecule Inhibitor
- In vitro assay of primary astrocyte migration as a tool to study Rho GTPase function in cell polarization
- Real-time centrosome reorientation during fibroblast migration
- Lentiviral delivery of RNAi in hippocampal neurons
- Methods for studying neutrophil chemotaxis
- Measurement of epidermal growth factor receptor turnover and effects of Cdc42
- Tumor cell migration in three dimensions
- An in vitro model to study the role of endothelial Rho GTPases during leukocyte transendothelial migration
- Analysis of a mitotic role of Cdc42
- Plexin-induced collapse assay in COS cells
- Morphological and biochemical analysis of Rac1 in three-dimensional epithelial cell cultures
- Using three-dimensional acinar structures for molecular and cell biological assays
- TC10 and insulin-stimulated glucose transport
- GTPases and the control of neuronal polarity
- In vitro assembly of filopodia-like bundles.
