Thiol redox transitions in cell signaling. Part A, Chemistry and biochemistry of low molecular weight and protein thiols /

• Άλλοι συγγραφείς: Cadenas, Enrique, Packer, Lester
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam, Netherlands ; Boston, Mass. : Elsevier/Academic, ©2010.
• Σειρά: Methods in enzymology ; volume 473.
• Θέματα: Cellular signal transduction.; Oxidation-reduction reaction.; SCIENCE > Life Sciences > Anatomy & Physiology.; Signal Transduction [MESH]; Oxidation-Reduction [MESH]; Enzymes [MESH]; Electronic books.

This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This firstinstallment of Cadenas and Packer's two-volume treatment specifically deals with glutathionylati...

Πίνακας περιεχομένων:

• Changing paradigms in thiology: from antioxidant defense toward redox regulation / Leopold Flohé
• Mass spectrometry-based methods for the determination of sulfur and related metabolite concentrations in cell extracts / Emmanuel Godat [and others]
• Use of dimedone-based chemical probes for sulfenic acid detection: evaluation of conditions affecting probe incorporation into redox-sensitive proteins / Chananat Klomsiri [and others]
• Use of dimedone-based chemical probes for sulfenic acid detection: methods to visualize and identify labeled proteins / Kimberly J. Nelson [and others]
• Formation and reactions of sulfenic acid in human serum albumin / Beatriz Alvarez [and others]
• Determination of GSH, GSSG, and GSNO using HPLC with electrochemical detection / Li-Peng Yap [and others]
• Measurement of mixed disulfides including glutathionylated proteins / Raffaella Priora [and others]
• Detection and quantification of protein disulfides in biological tissues: a fluorescence-based proteomic approach / Viviana I. Pérez [and others]
• Measurement and identification of S-glutathiolated proteins / Bradford G. Hill [and others]
• Proteome screens for Cys residues oxidation: the redoxome / Giovanni Chiappetta [and others]
• Identification by MS/MS of disulfides produced by a functional redox transition / Pierluigi Mauri [and others]
• Mass spectrometry approaches for the redox characterization of protein cysteine residues: the case of the transcription factor Pax-8 / Andrea Scaloni and Gianluca Tell
• A simple method to systematically study oxidatively modified proteins in biological samples and its applications / Byoung-Joon Song, Soo-Kyung Suh and Kwan-Hoon Moon
• Direct and indirect detection methods for the analysis of S-nitrosylated peptides and proteins / Federico Torta, Lisa Elviri and Angela Bachi
• A rapid approach for the detection, quantification, and discovery of novel sulfenic acid or S-nitrosothiol modified proteins using a biotin-switch method / Joseph R. Burgoyne and Philip Eaton
• Protein addicts of aldehydic lipid peroxidation products: identification and characterization of protein adducts using an aldehyde/keto-reactive probe in combination with mass spectrometry / Claudia S. Maier [and others].