Neurons : Methods and Applications for the Cell Biologist /

• Other Authors: Hollenbeck, Peter James (Editor), Bamburg, James R. (Editor)
• Format: eBook
• Language: English
• Published: San Diego : Academic Press, 2003.
• Series: Methods in cell biology ; v. 71.
• Subjects: Neurons.; Cytology.; Neurons > cytology.; Cell Culture Techniques > methods.; Cytological Techniques.; Neurones > Cytologie.; Cellules > Culture.; Cytologie > Technique.; Electronic books.
• Physical Description: 1 online resource (xvi, 441 pages, 14 unnumbered pages of plates) : illustrations.
• Bibliography: Includes bibliographical references and index.
• ISBN: 0123525659; 9780123525659

This book lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. * Contains culture methodology for more than a dozen typ.