Neurons : Methods and Applications for the Cell Biologist /

• Other Authors: Hollenbeck, Peter James (Editor), Bamburg, James R. (Editor)
• Format: eBook
• Language: English
• Published: San Diego : Academic Press, 2003.
• Series: Methods in cell biology ; v. 71.
• Subjects: Neurons.; Cytology.; Neurons > cytology.; Cell Culture Techniques > methods.; Cytological Techniques.; Neurones > Cytologie.; Cellules > Culture.; Cytologie > Technique.; Electronic books.

 This book lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. * Contains culture methodology for more than a dozen typ. 

 1. Comparing the properties of neuronal culture systems: a shopping guide for the cell biologist / Peter J. Hollenbeck and James R. Bamburg -- 2. Growing and working with peripheral neurons / Yan He and Peter W. Baas -- 3. Dissection and culturing of chick ciliary ganglion neurons: a system well suited to synaptic study / Barbara W. Bernstein -- 4. The culture of chick forebrain neurons / Steven R. Heidemann [and others] -- 5. Growing and working with spinal motor neurons / Thomas B. Kuhn -- 6. Avian purkinje neuronal cultures: extrinsic control of morphology by cell type and glutamate / Peter L. Jeffrey [and others] -- 7. Culturing hippocampal and cortical neurons / Peter J. Meberg and Matthew W. Miller -- 8. Working with Xenopus spinal neurons in live cell culture / Timothy M. Gómez [and others] -- 9. Culturing neurons from the snail Helisoma / Christopher S. Cohan, James L. Karnes, and Feng-Quan Zhou -- 10. The tibial-1 pioneer pathway: an in vivo model for neuronal outgrowth and guidance / Jennifer Bonner, Kimberly A. Gerrow, and Timothy P. O'Connor -- 11. Techniques to dissect cellular and subcellular function in the Drosophila nervous system / Heinrich J.G. Matthies and Kendal Broadie -- 12. PC12 cells as a model for studies of regulated secretion in neuronal and endocrine cells / T.F.J. Martin and R.N. Grishanin --13. B35 neuroblastoma cells: an easily transfected, cultured cell model of central nervous system neurons / Carol A. Otey, Malika Boukhelifa, and Patricia Maness -- 14. Live-cell imaging of slow axonal transport in cultured neurons / Anthony Brown -- 15. Making proteins into drugs: assisted delivery of proteins and peptides into living neurons / Gianluca Gallo -- 16. Transfection of primary central and peripheral nervous system neurons by electroporation / Cecilia Y. Martinez and Peter J. Hollenbeck -- 17. Biolistic transfection / Paul C. Bridgman, Michael E. Brown, and Irina Balan -- 18. Expression of transgenes in primary neurons from chick peripheral and central nervous systems by retroviral infection of early embryos / Peter J. Hollenbeck and D.M. Fekete -- 19. Production and use of replication-deficient adenovirus for transgene expression in neurons / L.S. Minamide [and others].