Cryo-EM. Part A, Sample preparation and data collection /

• Άλλοι συγγραφείς: Jensen, Grant J.
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: San Diego, Calif : Academic Press/Elsevier, Ã2010.
• Σειρά: Methods in enzymology ; v. 481.
• Θέματα: Electron microscopy > Technique.; Proteins > Structure.; Genomics.; SCIENCE > Electron Microscopes & Microscopy.; Microscopy, Electron.; Electronic books.

 This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. * Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide. 

 Part A: Sample preparation and data collection. 3d reconstruction from electron micrographs a personal account of its development -- Preparation of 2d crystals of membrane proteins for high-resolution electron crystallography data collection -- Helical crystallization of soluble and membrane binding proteins -- Plunge freezing for electron cryomicroscopy -- A practical guide to the use of monolayer purification and affinity grids -- Grafix: Stabilization of fragile macromolecular complexes for single particle cryo-EM -- Cryonegative staining of macromolecular assemblies -- Liposomes on a streptavidin crystal: A system to study membrane proteins by cryo-EM -- Micromanipulator-assisted vitreous cryosectioning and sample preparation by high-pressure freezing -- Site-specific biomolecule labeling with gold clusters -- How to operate a cryo-electron microscope -- Collecting electron crystallographic data of two-dimensional protein crystals -- Automated data collection for electron microscopic tomography -- Correlated light and electron cryo-microscopy -- Phase plates for transmission electron microscopy -- Radiation damage in electron cryomicroscopy.