Guide to techniques in mouse development. Part B, Mouse molecular genetics /

• Άλλοι συγγραφείς: Wassarman, Paul M., Soriano, Philippe M., 1953-
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam ; Boston : Elsevier /Academic Press, 2010.
• Έκδοση: 1st ed.
• Σειρά: Methods in enzymology ; v. 477.
• Θέματα: Mice > Cytology.; Mice > Embryology.; Mice > Development.; SCIENCE > Life Sciences > Cell Biology.; Mice > growth & development.; Mice > embryology.; Cell Line.; Embryonic Stem Cells > cytology.; Electronic books.

 This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. Comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap. 

 Part B. Lentivirus transgenesis -- Lentivirus transgenesis -- Germline modification using mouse spermatogonial stem cells -- Embryonic in vivo electroporation in the mouse -- Current applications of transposons in mouse genetics -- Functional genomics in the mouse using the sleeping beauty transposon system -- The use of DNA transposons for cancer gene discovery in mice -- A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of creert2 -- A recombineering pipeline to make conditional targeting constructs -- Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria -- Genetic fate mapping using site-specific recombinases -- Mapping cell fate and function using recombinase-based intersectional strategies -- Genome-wide forward genetic screens in mouse es cells -- Gene trap mutagenesis in the mouse -- A wider context for gene trap mutagenesis -- Mouse mutagenesis with the chemical supermutagen enu -- Phenotype-driven mouse enu mutagenesis screens -- Using enu mutagenesis for phenotype-driven analysis of the mouse -- Exploration of self-renewal and pluripotency in es cells using rnai -- Transgenic rnai applications in the mouse -- Gene knockdown in the mouse through rnai -- In vivo analysis of gene knockdown in tetracycline-inducible shrna mice -- The power of reversibility regulating gene activities via tetracycline-controlled transcription -- Gene expression profiling of mouse oocytes and preimplantation embryos -- Interrogating the transcriptome of oocytes and preimplantation embryos -- Gene expression profiling of mouse embryos with microarrays -- Technological advances in mouse development studies.