Guide to techniques in mouse development. Part B, Mouse molecular genetics /

• Άλλοι συγγραφείς: Wassarman, Paul M., Soriano, Philippe M., 1953-
• Μορφή: Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Amsterdam ; Boston : Elsevier /Academic Press, 2010.
• Έκδοση: 1st ed.
• Σειρά: Methods in enzymology ; v. 477.
• Θέματα: Mice > Cytology.; Mice > Embryology.; Mice > Development.; SCIENCE > Life Sciences > Cell Biology.; Mice > growth & development.; Mice > embryology.; Cell Line.; Embryonic Stem Cells > cytology.; Electronic books.

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. Comprehensivel...

Πίνακας περιεχομένων:

• Part B. Lentivirus transgenesis
• Lentivirus transgenesis
• Germline modification using mouse spermatogonial stem cells
• Embryonic in vivo electroporation in the mouse
• Current applications of transposons in mouse genetics
• Functional genomics in the mouse using the sleeping beauty transposon system
• The use of DNA transposons for cancer gene discovery in mice
• A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of creert2
• A recombineering pipeline to make conditional targeting constructs
• Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria
• Genetic fate mapping using site-specific recombinases
• Mapping cell fate and function using recombinase-based intersectional strategies
• Genome-wide forward genetic screens in mouse es cells
• Gene trap mutagenesis in the mouse
• A wider context for gene trap mutagenesis
• Mouse mutagenesis with the chemical supermutagen enu
• Phenotype-driven mouse enu mutagenesis screens
• Using enu mutagenesis for phenotype-driven analysis of the mouse
• Exploration of self-renewal and pluripotency in es cells using rnai
• Transgenic rnai applications in the mouse
• Gene knockdown in the mouse through rnai
• In vivo analysis of gene knockdown in tetracycline-inducible shrna mice
• The power of reversibility regulating gene activities via tetracycline-controlled transcription
• Gene expression profiling of mouse oocytes and preimplantation embryos
• Interrogating the transcriptome of oocytes and preimplantation embryos
• Gene expression profiling of mouse embryos with microarrays
• Technological advances in mouse development studies.